Cochlear dopamine release is modulated by group II metabotropic glutamate receptors via GABAergic neurotransmission

Zoltán Doleviczényi, György Halmos, Gábor Répássy, E. Sylvester Vizi, Tibor Zelles, Balázs Lendvai

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Dopamine (DA), released from the lateral olivocochlear efferent fibers, is suggested to be neuroprotective against ischemia and noise exposure in the mammalian cochlea because it can reduce the postsynaptic excitotoxic effect of glutamate on the dendrite of the afferent auditory neuron. Using in vitro microvolume superfusion method on isolated guinea pig cochlea preparation, we found that the selective mGluR2/3 agonist (2R,4R)-aminopyrrolidine-2,4- dicarboxylic acid (2R,4R-APDC) significantly increased the release of DA in a dose-dependent manner. Other mGluR agonists, acting on groups I and III receptors (3,5-dihydroxyphenylglycine, amino-4-phosphonobutyric acid) and antagonists (2-methyl-6-(phenylethynyl)pyridine), (2S)-2-amino-2-(1S,2S-2- carboxycyclopronan-1-yl-3-(xanth9-yl)propanoic acid, α-methylserine-O- phosphate), were ineffective. The GABAA antagonist bicuculline (10 μM) could antagonize the effect of 2R,4R-APDC suggesting that the mGluR-mediated enhancement of DA release was most likely attributable to a disinhibitory mechanism involving local GABAergic fibers. Bicuculline alone could also elevate the DA outflow indicating that cochlear GABA controls local DA release tonically. Our findings expand the view on the local effects of glutamate in the cochlea by showing the ability of the excitatory neurotransmitter to alleviate its own action on type I afferents via mGluRs and initiate a neuroprotective mechanism.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalNeuroscience Letters
Volume385
Issue number2
DOIs
Publication statusPublished - Sep 9 2005

Fingerprint

Metabotropic Glutamate Receptors
Cochlea
Synaptic Transmission
Dopamine
Dicarboxylic Acids
Bicuculline
Glutamic Acid
GABA-A Receptor Antagonists
Afferent Neurons
Dendrites
gamma-Aminobutyric Acid
Neurotransmitter Agents
Noise
Guinea Pigs
Ischemia
Acids

Keywords

  • Dopamine release
  • GABA
  • Lateral olivocochlear efferents
  • mGluR

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cochlear dopamine release is modulated by group II metabotropic glutamate receptors via GABAergic neurotransmission. / Doleviczényi, Zoltán; Halmos, György; Répássy, Gábor; Vizi, E. Sylvester; Zelles, Tibor; Lendvai, Balázs.

In: Neuroscience Letters, Vol. 385, No. 2, 09.09.2005, p. 93-98.

Research output: Contribution to journalArticle

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