Coating with autologous plasma improves biocompatibility of mesh grafts in vitro: Development stage of a surgical innovation

Holger Gerullis, Evangelos Georgas, Christoph Eimer, Christian Arndt, Dimitri Barski, Bernhard Lammers, Bernd Klosterhalfen, M. Borós, Thomas Otto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose. To investigate mesh coating modalities with autologous blood components in a recently developed in vitro test system for biocompatibility assessment of alloplastic materials. Materials and Methods. Seven different mesh types, currently used in various indications, were randomly investigated. Meshes were coated prior to cultivation with autologous peripheral blood mononuclear cells (PBMCs), platelets, and blood plasma. Pretreated meshes were incubated over 6 weeks in a minced tissue assay, representative for fibroblasts, muscle cells, and endothelial cells originating from 10 different patients. Adherence of those tissues on the meshes was microscopically investigated and semiquantitatively assessed using a previously described scoring system. Results. Coating with peripheral blood mononuclear cells did not affect the adherence score, whereas coating with platelets and blood plasma increased the score suggesting improved biocompatibility in vitro. The previous ranking of native meshes remained consistent after coating. Conclusion. Plasma coating of meshes improves their biocompatibility score in a novel in vitro test system.

Original languageEnglish
Article number536814
JournalBioMed Research International
Volume2013
DOIs
Publication statusPublished - 2013

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Biocompatibility
Grafts
Blood
Innovation
Transplants
Plasmas
Coatings
Blood Cells
Blood Platelets
Platelets
Muscle Cells
Tissue
Endothelial Cells
Fibroblasts
Endothelial cells
Muscle
Assays
Cells
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Coating with autologous plasma improves biocompatibility of mesh grafts in vitro : Development stage of a surgical innovation. / Gerullis, Holger; Georgas, Evangelos; Eimer, Christoph; Arndt, Christian; Barski, Dimitri; Lammers, Bernhard; Klosterhalfen, Bernd; Borós, M.; Otto, Thomas.

In: BioMed Research International, Vol. 2013, 536814, 2013.

Research output: Contribution to journalArticle

Gerullis, Holger ; Georgas, Evangelos ; Eimer, Christoph ; Arndt, Christian ; Barski, Dimitri ; Lammers, Bernhard ; Klosterhalfen, Bernd ; Borós, M. ; Otto, Thomas. / Coating with autologous plasma improves biocompatibility of mesh grafts in vitro : Development stage of a surgical innovation. In: BioMed Research International. 2013 ; Vol. 2013.
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AU - Georgas, Evangelos

AU - Eimer, Christoph

AU - Arndt, Christian

AU - Barski, Dimitri

AU - Lammers, Bernhard

AU - Klosterhalfen, Bernd

AU - Borós, M.

AU - Otto, Thomas

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