Coamplified and overexpressed genes at ERBB2 locus in gastric cancer

Asta Varis, Alexander Zaika, Pauli Puolakkainen, Balint Nagy, Irene Madrigal, Arto Kokkola, Aki Väyrynen, Päivi Kärkkäinen, Christopher Moskaluk, Wa'el El-Rifai, Sakari Knuutila

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Abstract

DNA copy number amplification at the chromosomal region of 17q is frequent in gastric cancer. Recently 17q21 was identified as the critical region for the amplicon formation because this region harbors the ERBB2 oncogene and several other targets, such as TOP2A and DARPP32. In our study, we characterized the amplification (52 cases) and expression (29 cases) levels of ERBB2, TOP2A and DARPP32 in gastric cancer samples. These 3 genes were concomitantly amplified in 17% of the intestinal type of gastric adenocarcinoma. However, the expression levels were independent, showing overexpression of DARPP32 (48%), TOP2A (17%) and ERBB2 (3%) studied by quantitative real-time PCR. The most frequently overexpressed gene, DARPP32, exhibited strong protein overexpression in 45% (30/66) of the cases in immunohistochemical study of gastric cancer tumor tissue array. Additional studies are required to thoroughly understand the biological significance of these genes in gastric cancer.

Original languageEnglish
Pages (from-to)548-553
Number of pages6
JournalInternational Journal of Cancer
Volume109
Issue number4
DOIs
Publication statusPublished - Apr 20 2004

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Keywords

  • DARPP32
  • ERBB2
  • Gastric cancer
  • Gene amplification
  • TOP2A

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Varis, A., Zaika, A., Puolakkainen, P., Nagy, B., Madrigal, I., Kokkola, A., Väyrynen, A., Kärkkäinen, P., Moskaluk, C., El-Rifai, W., & Knuutila, S. (2004). Coamplified and overexpressed genes at ERBB2 locus in gastric cancer. International Journal of Cancer, 109(4), 548-553. https://doi.org/10.1002/ijc.20001