Coagulation factor XIII-A. A flow cytometric intracellular marker in the classification of acute myeloid leukemias

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Abstract

The association of coagulation factors with leukocytes have been demonstrated in several previous studies. This study was designed to study the sensitivity and specificity of factor XIII subunitA (FXIII-A) labelling in cultured myeloblastic and monoblastic cell lines and to investigate the intracytoplasmic expression of FXIII-A in de novo acute myeloid leukemia (AML) samples. Myeloblastic and a monoblastic cell lines were cultured and investigated for lineage specific maturation markers and FXIII-A expression. Furthermore, FXIII-A expression was investigated in 12 normal samples (7 bone marrow and 5 peripheral blood), 86 de novo AML samples and 6 chronic myelomonocytic leukemia (CMML) samples. In the monoblastic MonoMac6 cell line the appearance of FXIII-A preceded that of CD14 while it remained negative in the myeloblastic PLB-985 cell line throughout its maturation period. Among the AML samples the average frequency of FXIII-A positive cells in myeloblastic leukemia samples was below 10%, while in M4 and M5 AML samples it was above 50% and was significantly higher than the generally used CD14 marker (p<0.0001). In the AML M4 and M5 cases, FXIII-A proved sensitive for the identification of monoblasts. FXIII-A can be considered as a reliable intracytoplasmic marker for the monocytic and megakaryocytic series and its presence is highly predictive for mono- and megakaryocytic AML and for CMML.

Original languageEnglish
Pages (from-to)454-459
Number of pages6
JournalThrombosis and Haemostasis
Volume94
Issue number2
DOIs
Publication statusPublished - Aug 1 2005

Keywords

  • Acute myeloid leukemia classification
  • Factor XIII
  • Flow cytometry

ASJC Scopus subject areas

  • Hematology

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