Co-transfer of embryos derived from cryopreserved and fresh natural cycle oocytes: A pilot study

Ermanno Greco, K. Litwicka, S. Ferrero, F. Sapienza, M. G. Minasi, M. Iacobelli, D. Zavaglia, P. Nagy

Research output: Contribution to journalArticle

Abstract

Italian legislation regarding reproductive medicine prohibits embryo storage while allowing cryopreservation of supernumerary oocytes. This study evaluated the effect of fresh oocytes obtained from natural unstimulated cycles on the clinical success rates derived from the use of frozen-thawed (FR-TH) oocytes obtained following ovarian stimulation. For 36 women, intracytoplasmic sperm injection was performed on FR-TH oocytes supplemented by a fresh oocyte, if available, derived from a natural cycle in which gonadotrophin-releasing hormone-antagonist was used for premature LH surge control. The retrieval rate of fresh oocytes was 61.1% and survival rate of FR-TH oocytes was 43.6%. The fertilization rate of fresh and FR-TH oocytes was 70% and 52.5%, respectively. Fifty embryos were transferred, 14 of them developed from fresh oocytes and 36 from FR-TH oocytes. Six pregnancies occurred in 10 cycles in which the embryos developed from fresh and FR-TH oocytes (pregnancy rate 60.0%) and two in 12 patients in whom the embryos were obtained from only FR-TH oocytes (pregnancy rate 16.7%) (P <0.05). In summary, the data demonstrate that the transfer of embryos derived from oocytes cryopreserved following a previous ovarian stimulation and an embryo developed from a fresh one retrieved in natural cycle ensures an excellent clinical outcome.

Original languageEnglish
Pages (from-to)530-536
Number of pages7
JournalReproductive BioMedicine Online
Volume17
Issue number4
Publication statusPublished - Oct 2008

Fingerprint

Embryo Transfer
Oocytes
Embryonic Structures
Ovulation Induction
Pregnancy Rate
Reproductive Medicine
Hormone Antagonists
Intracytoplasmic Sperm Injections
Cryopreservation
Legislation
Fertilization
Gonadotropin-Releasing Hormone
Survival Rate

Keywords

  • Clinical efficacy
  • ICSI
  • Natural cycle
  • Oocyte cryopreservation
  • Oocyte restriction

ASJC Scopus subject areas

  • Reproductive Medicine
  • Developmental Biology

Cite this

Greco, E., Litwicka, K., Ferrero, S., Sapienza, F., Minasi, M. G., Iacobelli, M., ... Nagy, P. (2008). Co-transfer of embryos derived from cryopreserved and fresh natural cycle oocytes: A pilot study. Reproductive BioMedicine Online, 17(4), 530-536.

Co-transfer of embryos derived from cryopreserved and fresh natural cycle oocytes : A pilot study. / Greco, Ermanno; Litwicka, K.; Ferrero, S.; Sapienza, F.; Minasi, M. G.; Iacobelli, M.; Zavaglia, D.; Nagy, P.

In: Reproductive BioMedicine Online, Vol. 17, No. 4, 10.2008, p. 530-536.

Research output: Contribution to journalArticle

Greco, E, Litwicka, K, Ferrero, S, Sapienza, F, Minasi, MG, Iacobelli, M, Zavaglia, D & Nagy, P 2008, 'Co-transfer of embryos derived from cryopreserved and fresh natural cycle oocytes: A pilot study', Reproductive BioMedicine Online, vol. 17, no. 4, pp. 530-536.
Greco E, Litwicka K, Ferrero S, Sapienza F, Minasi MG, Iacobelli M et al. Co-transfer of embryos derived from cryopreserved and fresh natural cycle oocytes: A pilot study. Reproductive BioMedicine Online. 2008 Oct;17(4):530-536.
Greco, Ermanno ; Litwicka, K. ; Ferrero, S. ; Sapienza, F. ; Minasi, M. G. ; Iacobelli, M. ; Zavaglia, D. ; Nagy, P. / Co-transfer of embryos derived from cryopreserved and fresh natural cycle oocytes : A pilot study. In: Reproductive BioMedicine Online. 2008 ; Vol. 17, No. 4. pp. 530-536.
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