Co-ordination of the nitrate and nitrite assimilation, glutathione and free radical metabolisms, and the pentose phosphate pathway in Penicillium chrysogenum

T. Emri, László Sámi, A. Szentirmai, I. Pócsi

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13 Citations (Scopus)

Abstract

The specific glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD) activities increased significantly in Penicillium chrysogenum grown on glucose in the presence of either nitrate or nitrite. These changes kept the intracellular peroxide levels low and prevented the onset of any glutathione/glutathione disulphide redox imbalances. On the other hand, the specific activity of several other glutathione metabolic enzymes including glutathione peroxidase, glutathione S-transferase, γ-glutamyltranspeptidase and the glutathione producing activity was not effected by NO3/- and NO2/-. When P. chrysogenum mycelia were challenged with oxidative stress caused by high concentrations of H2O2, tert-butyl hydroperoxide, menadione, diamide or phenoxyacetic acid the intracellular peroxide concentrations increased significantly, and the nitrate reductase and nitrite reductase activities were eliminated. When glucose was replaced with lactose the GR and G6PD activities were not influenced by the nitrogen sources but in this case the intracellular GSH concentration was twice higher than that observed with glucose.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalJournal of Basic Microbiology
Volume39
Issue number2
DOIs
Publication statusPublished - 1999

Fingerprint

Penicillium chrysogenum
Pentose Phosphate Pathway
Nitrites
Nitrates
Free Radicals
Glutathione
Glutathione Reductase
Glucosephosphate Dehydrogenase
Peroxides
Glucose
Nitrite Reductases
Diamide
tert-Butylhydroperoxide
Vitamin K 3
Nitrate Reductase
Glutathione Disulfide
Mycelium
Lactose
Glutathione Peroxidase
Glutathione Transferase

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

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title = "Co-ordination of the nitrate and nitrite assimilation, glutathione and free radical metabolisms, and the pentose phosphate pathway in Penicillium chrysogenum",
abstract = "The specific glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD) activities increased significantly in Penicillium chrysogenum grown on glucose in the presence of either nitrate or nitrite. These changes kept the intracellular peroxide levels low and prevented the onset of any glutathione/glutathione disulphide redox imbalances. On the other hand, the specific activity of several other glutathione metabolic enzymes including glutathione peroxidase, glutathione S-transferase, γ-glutamyltranspeptidase and the glutathione producing activity was not effected by NO3/- and NO2/-. When P. chrysogenum mycelia were challenged with oxidative stress caused by high concentrations of H2O2, tert-butyl hydroperoxide, menadione, diamide or phenoxyacetic acid the intracellular peroxide concentrations increased significantly, and the nitrate reductase and nitrite reductase activities were eliminated. When glucose was replaced with lactose the GR and G6PD activities were not influenced by the nitrogen sources but in this case the intracellular GSH concentration was twice higher than that observed with glucose.",
author = "T. Emri and L{\'a}szl{\'o} S{\'a}mi and A. Szentirmai and I. P{\'o}csi",
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AU - Emri, T.

AU - Sámi, László

AU - Szentirmai, A.

AU - Pócsi, I.

PY - 1999

Y1 - 1999

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AB - The specific glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD) activities increased significantly in Penicillium chrysogenum grown on glucose in the presence of either nitrate or nitrite. These changes kept the intracellular peroxide levels low and prevented the onset of any glutathione/glutathione disulphide redox imbalances. On the other hand, the specific activity of several other glutathione metabolic enzymes including glutathione peroxidase, glutathione S-transferase, γ-glutamyltranspeptidase and the glutathione producing activity was not effected by NO3/- and NO2/-. When P. chrysogenum mycelia were challenged with oxidative stress caused by high concentrations of H2O2, tert-butyl hydroperoxide, menadione, diamide or phenoxyacetic acid the intracellular peroxide concentrations increased significantly, and the nitrate reductase and nitrite reductase activities were eliminated. When glucose was replaced with lactose the GR and G6PD activities were not influenced by the nitrogen sources but in this case the intracellular GSH concentration was twice higher than that observed with glucose.

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