Co-localization of serotonin and FMRFamide-like immunoreactivities in the central nervous system of the earthworm, Eisenia fetida

A. Lubics, D. Reglodi, Sándor Slezák, Márta Szelier, I. Lengvári

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In the present study co-localization of serotonin and FMRFamide-like immunoreactivities was studied in the central nervous system of the earthworm, Eisenia fetida. Alternate serial sections were stained with antisera raised against serotonin and FMRFamide, using the peroxidase- antiperoxidase method. The immunostained consecutive sections were compared by light-microscopy and on reconstructed images using NIH Image and Neurolucida programs. Although there was a large number of serotonin and FMRFamide-like immunoreactive neurons in the central nervous system of Eisenia, co-localization of immunostaining was relatively rare. In the cerebral ganglion no cells were found that stained for both antisera. In the subesophageal and ventral cord ganglia the neurons reacting for both antisera represented approximately 17-23% and 10-14% of the serotonin- and 7-11% and 9-17% of the FMRFamide-like immunoreactive cells, respectively. It is suggested, that FMRFamide-related peptides act as neuromodulators and may also influence the activity of serotonergic neurons.

Original languageEnglish
Pages (from-to)459-467
Number of pages9
JournalActa Histochemica
Volume99
Issue number4
Publication statusPublished - 1997

Fingerprint

FMRFamide
Oligochaeta
Serotonin
Central Nervous System
Immune Sera
Ganglia
Serotonergic Neurons
Neurons
Peroxidase
Neurotransmitter Agents
Microscopy
Light
Peptides

Keywords

  • Earthworm FMRFamide
  • Eisenia letida
  • Immunohistochemistry
  • Oligochatea
  • Serotonin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Co-localization of serotonin and FMRFamide-like immunoreactivities in the central nervous system of the earthworm, Eisenia fetida. / Lubics, A.; Reglodi, D.; Slezák, Sándor; Szelier, Márta; Lengvári, I.

In: Acta Histochemica, Vol. 99, No. 4, 1997, p. 459-467.

Research output: Contribution to journalArticle

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AU - Slezák, Sándor

AU - Szelier, Márta

AU - Lengvári, I.

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AB - In the present study co-localization of serotonin and FMRFamide-like immunoreactivities was studied in the central nervous system of the earthworm, Eisenia fetida. Alternate serial sections were stained with antisera raised against serotonin and FMRFamide, using the peroxidase- antiperoxidase method. The immunostained consecutive sections were compared by light-microscopy and on reconstructed images using NIH Image and Neurolucida programs. Although there was a large number of serotonin and FMRFamide-like immunoreactive neurons in the central nervous system of Eisenia, co-localization of immunostaining was relatively rare. In the cerebral ganglion no cells were found that stained for both antisera. In the subesophageal and ventral cord ganglia the neurons reacting for both antisera represented approximately 17-23% and 10-14% of the serotonin- and 7-11% and 9-17% of the FMRFamide-like immunoreactive cells, respectively. It is suggested, that FMRFamide-related peptides act as neuromodulators and may also influence the activity of serotonergic neurons.

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