Abstract
In the present study co-localization of serotonin and FMRFamide-like immunoreactivities was studied in the central nervous system of the earthworm, Eisenia fetida. Alternate serial sections were stained with antisera raised against serotonin and FMRFamide, using the peroxidase- antiperoxidase method. The immunostained consecutive sections were compared by light-microscopy and on reconstructed images using NIH Image and Neurolucida programs. Although there was a large number of serotonin and FMRFamide-like immunoreactive neurons in the central nervous system of Eisenia, co-localization of immunostaining was relatively rare. In the cerebral ganglion no cells were found that stained for both antisera. In the subesophageal and ventral cord ganglia the neurons reacting for both antisera represented approximately 17-23% and 10-14% of the serotonin- and 7-11% and 9-17% of the FMRFamide-like immunoreactive cells, respectively. It is suggested, that FMRFamide-related peptides act as neuromodulators and may also influence the activity of serotonergic neurons.
| Original language | English |
|---|---|
| Pages (from-to) | 459-467 |
| Number of pages | 9 |
| Journal | Acta Histochemica |
| Volume | 99 |
| Issue number | 4 |
| Publication status | Published - 1997 |
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Keywords
- Earthworm FMRFamide
- Eisenia letida
- Immunohistochemistry
- Oligochatea
- Serotonin
ASJC Scopus subject areas
- Cell Biology
Cite this
Co-localization of serotonin and FMRFamide-like immunoreactivities in the central nervous system of the earthworm, Eisenia fetida. / Lubics, A.; Reglodi, D.; Slezák, Sándor; Szelier, Márta; Lengvári, I.
In: Acta Histochemica, Vol. 99, No. 4, 1997, p. 459-467.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Co-localization of serotonin and FMRFamide-like immunoreactivities in the central nervous system of the earthworm, Eisenia fetida
AU - Lubics, A.
AU - Reglodi, D.
AU - Slezák, Sándor
AU - Szelier, Márta
AU - Lengvári, I.
PY - 1997
Y1 - 1997
N2 - In the present study co-localization of serotonin and FMRFamide-like immunoreactivities was studied in the central nervous system of the earthworm, Eisenia fetida. Alternate serial sections were stained with antisera raised against serotonin and FMRFamide, using the peroxidase- antiperoxidase method. The immunostained consecutive sections were compared by light-microscopy and on reconstructed images using NIH Image and Neurolucida programs. Although there was a large number of serotonin and FMRFamide-like immunoreactive neurons in the central nervous system of Eisenia, co-localization of immunostaining was relatively rare. In the cerebral ganglion no cells were found that stained for both antisera. In the subesophageal and ventral cord ganglia the neurons reacting for both antisera represented approximately 17-23% and 10-14% of the serotonin- and 7-11% and 9-17% of the FMRFamide-like immunoreactive cells, respectively. It is suggested, that FMRFamide-related peptides act as neuromodulators and may also influence the activity of serotonergic neurons.
AB - In the present study co-localization of serotonin and FMRFamide-like immunoreactivities was studied in the central nervous system of the earthworm, Eisenia fetida. Alternate serial sections were stained with antisera raised against serotonin and FMRFamide, using the peroxidase- antiperoxidase method. The immunostained consecutive sections were compared by light-microscopy and on reconstructed images using NIH Image and Neurolucida programs. Although there was a large number of serotonin and FMRFamide-like immunoreactive neurons in the central nervous system of Eisenia, co-localization of immunostaining was relatively rare. In the cerebral ganglion no cells were found that stained for both antisera. In the subesophageal and ventral cord ganglia the neurons reacting for both antisera represented approximately 17-23% and 10-14% of the serotonin- and 7-11% and 9-17% of the FMRFamide-like immunoreactive cells, respectively. It is suggested, that FMRFamide-related peptides act as neuromodulators and may also influence the activity of serotonergic neurons.
KW - Earthworm FMRFamide
KW - Eisenia letida
KW - Immunohistochemistry
KW - Oligochatea
KW - Serotonin
UR - http://www.scopus.com/inward/record.url?scp=0030833950&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030833950&partnerID=8YFLogxK
M3 - Article
C2 - 9429604
AN - SCOPUS:0030833950
VL - 99
SP - 459
EP - 467
JO - Acta Histochemica
JF - Acta Histochemica
SN - 0065-1281
IS - 4
ER -