Co-immobilized Whole Cells with ω-Transaminase and Ketoreductase Activities for Continuous-Flow Cascade Reactions

László Nagy-Győr, Emese Abaházi, Viktória Bódai, Péter Sátorhelyi, Balázs Erdélyi, Diána Balogh-Weiser, Csaba Paizs, Gábor Hornyánszky, L. Poppe

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An improved sol–gel process involving the use of hollow silica microspheres as a supporting additive was applied for the co-immobilization of whole cells of Escherichia coli with Chromobacterium violaceum ω-transaminase activity and Lodderomyces elongisporus with ketoreductase activity. The co-immobilized cells with two different biocatalytic activities could perform a cascade of reactions to convert racemic 4-phenylbutan-2-amine or heptan-2-amine into a nearly equimolar mixture of the corresponding enantiomerically pure R amine and S alcohol even in continuous-flow mode. The novel co-immobilized whole-cell system proved to be an easy-to-store and durable biocatalyst.

Original languageEnglish
Pages (from-to)1845-1848
Number of pages4
JournalChemBioChem
Volume19
Issue number17
DOIs
Publication statusPublished - Sep 4 2018

Keywords

  • continuous flow
  • kinetic resolution
  • oxidoreductases
  • transferases
  • whole-cell immobilization

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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  • Cite this

    Nagy-Győr, L., Abaházi, E., Bódai, V., Sátorhelyi, P., Erdélyi, B., Balogh-Weiser, D., Paizs, C., Hornyánszky, G., & Poppe, L. (2018). Co-immobilized Whole Cells with ω-Transaminase and Ketoreductase Activities for Continuous-Flow Cascade Reactions. ChemBioChem, 19(17), 1845-1848. https://doi.org/10.1002/cbic.201800286