Co-expression of human ABCG5 and ABCG8 in insect cells generates an androstan stimulated membrane ATPase activity

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Abstract

Mutations in the ATP-binding cassette (ABC) proteins ABCG5 or ABCG8 cause sitosterolemia, a condition with increased accumulation of plant sterols. Upon high level expression of the ABCG5 and ABCG8 proteins in baculovirus-Sf9 cell expression system we found a distinct, vanadate sensitive ATPase activity in isolated membrane preparations only when the two proteins were co-expressed. This ATPase activity was significantly stimulated by the addition of certain androgen hormones and analogs, and was effectively inhibited by progesterone. Our results provide a new aspect of biochemical and functional characterization of the ABCG5/ABCG8 proteins and their possible involvement in steroid hormone transport or regulation.

Original languageEnglish
Pages (from-to)6139-6144
Number of pages6
JournalFEBS letters
Volume580
Issue number26
DOIs
Publication statusPublished - Nov 13 2006

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Keywords

  • ATP binding cassette transporters
  • Sitosterolemia
  • Steroid hormones

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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