Co-determination of ATP and proteins in Triton X 100 non-ionic detergent-opened monolayer cultured cells

Tamás Köszegi, József Petrik, Sanda Vladimir-Knežević, Sándor Nagy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human monolayer cells (HEp-2 and Hep G2) were cultured in 96-well plates. A modified Triton X 100 nonionic detergent extraction method was used for releasing intracellular ATP and protein in one step. The detergent technique was compared to perchloric acid (PCA) extraction. ATP was determined by the firefly bioluminescence method and ATP values were referred to cell protein (ATP:protein ratio). There was no significant difference in ATP data between detergent and PCA treatments. The ATP:protein ratio seems to be a sensitive tool for characterizing the metabolic activity of monolayer tissue culture cells. The protein-mobilizing capability of Triton X 100 depends on the type of cell culture used. Our modified extraction gives reliable ATP:protein values with one simple extraction step.

Original languageEnglish
Pages (from-to)415-419
Number of pages5
JournalLuminescence
Volume22
Issue number5
DOIs
Publication statusPublished - Sep 1 2007

Keywords

  • ATP:protein ratio
  • Bioluminescence
  • Cell culture
  • Intracellular ATP
  • Triton X 100

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)

Fingerprint Dive into the research topics of 'Co-determination of ATP and proteins in Triton X 100 non-ionic detergent-opened monolayer cultured cells'. Together they form a unique fingerprint.

  • Cite this