Clusterin expression in cholestasis, hepatocellular carcinoma and liver fibrosis

Ariane Aigelsreiter, Elke Janig, Julia Sostaric, Martin Pichler, Daniela Unterthor, J. Halász, Carolin Lackner, Kurt Zatloukal, Helmut Denk

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aims: Clusterin shares functional properties with small heat shock proteins. In contrast to other heat shock proteins, it is present in the extracellular space. Its expression is altered in various diseases. The aim was to evaluate the presence and distribution of clusterin in liver diseases associated with cholestasis, in fibrosis and in hepatocellular carcinoma. Methods and results: Tissue microarrays and biopsy materials were used to evaluate immunohistochemically the expression of clusterin in hepatocellular carcinoma, primary sclerosing cholangitis, primary biliary cirrhosis, mechanical cholestasis, drug-induced cholestasis, liver fibrosis and cirrhosis. The presence of clusterin in human bile was assessed by Western blotting. Furthermore, real-time reverse transcriptase-polymerase chain reaction was performed on liver tissue with mechanical cholestasis. Clusterin colocalized with elastic fibres, but not with collagen, hepatocytes or bile duct epithelia. It was detected in bile plugs in cholestasis and hepatocellular carcinomas with pseudoglandular features within the lumina. Clusterin was demonstrated in bile by Western blotting and its mRNA was expressed in normal and cholestatic livers. Conclusions: Clusterin may protect bile duct epithelium against offensive biliary components or inhibit precipitation of biliary proteins. The association of clusterin with elastic fibres could reflect an extracellular chaperone function by either protecting elastic fibres or shielding abnormal elastic material.

Original languageEnglish
Pages (from-to)561-570
Number of pages10
JournalHistopathology
Volume54
Issue number5
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Clusterin
Cholestasis
Liver Cirrhosis
Hepatocellular Carcinoma
Elastic Tissue
Bile
Bile Ducts
Epithelium
Western Blotting
Small Heat-Shock Proteins
Sclerosing Cholangitis
Biliary Liver Cirrhosis
Liver
Extracellular Space
Heat-Shock Proteins
Reverse Transcriptase Polymerase Chain Reaction
Liver Diseases
Real-Time Polymerase Chain Reaction
Hepatocytes
Fibrosis

Keywords

  • Chaperones
  • Cholestasis
  • Cirrhosis
  • Clusterin
  • Elastic fibres
  • Heat shock proteins
  • Liver fibrosis

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Aigelsreiter, A., Janig, E., Sostaric, J., Pichler, M., Unterthor, D., Halász, J., ... Denk, H. (2009). Clusterin expression in cholestasis, hepatocellular carcinoma and liver fibrosis. Histopathology, 54(5), 561-570. https://doi.org/10.1111/j.1365-2559.2009.03258.x

Clusterin expression in cholestasis, hepatocellular carcinoma and liver fibrosis. / Aigelsreiter, Ariane; Janig, Elke; Sostaric, Julia; Pichler, Martin; Unterthor, Daniela; Halász, J.; Lackner, Carolin; Zatloukal, Kurt; Denk, Helmut.

In: Histopathology, Vol. 54, No. 5, 04.2009, p. 561-570.

Research output: Contribution to journalArticle

Aigelsreiter, A, Janig, E, Sostaric, J, Pichler, M, Unterthor, D, Halász, J, Lackner, C, Zatloukal, K & Denk, H 2009, 'Clusterin expression in cholestasis, hepatocellular carcinoma and liver fibrosis', Histopathology, vol. 54, no. 5, pp. 561-570. https://doi.org/10.1111/j.1365-2559.2009.03258.x
Aigelsreiter, Ariane ; Janig, Elke ; Sostaric, Julia ; Pichler, Martin ; Unterthor, Daniela ; Halász, J. ; Lackner, Carolin ; Zatloukal, Kurt ; Denk, Helmut. / Clusterin expression in cholestasis, hepatocellular carcinoma and liver fibrosis. In: Histopathology. 2009 ; Vol. 54, No. 5. pp. 561-570.
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