Cloning of the Rhizomucor miehe 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene and its heterologous expression in Mucor circinelloides

Gyöngyi Lukács, Tamás Papp, Ferenc Somogyvári, Árpád Csernetics, Ildikó Nyilasi, Csaba Vágvögyi

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11 Citations (Scopus)

Abstract

In this study, the gene hmgR encoding the 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) was cloned and characterized in the zygomycete fungus Rhizomucor miehei. The hmgR gene comprises a total of 3,585 bp including the coding sequence of a 1,058 amino acids length putative protein and five introns (137, 83, 59, 60 and 69 bp in length) dispersed in the whole coding region. Southern hybridization analysis revealed that the gene is present only in one copy in the R. miehei genome. The isolated Rhizomucor gene was expressed in the related fungus, Mucor circinelloides. Transformants harbouring the Rhizomucor hmgR gene in an autoreplicative plasmid proved to be more tolerant to statins (e.g. lovastatin, simvastatin, and fluvastatin), the competitive inhibitors of the HMG-CoA reductase, than the original M. circinelloides strain. At the same time, heterologous expression of the Rhizomucor hmgR did not affect the carotenoid production of M. circinelloides.

Original languageEnglish
Pages (from-to)55-64
Number of pages10
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume95
Issue number1
DOIs
Publication statusPublished - Jan 1 2009

Keywords

  • Carotenoid
  • HMG-coenzyme A reductase
  • Statin
  • Sterol

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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