Cloning of the bovine leukemia virus proteinase in Escherichia coli and comparison of its specificity to that of human T-cell leukemia virus proteinase

Gábor Zahuczky, Péter Boross, P. Bagossi, Gabriella Emri, Terry D. Copeland, Stephen Oroszlan, John M. Louis, J. Tőzsér

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The proteinase of bovine leukemia virus (BLV) was cloned into pMal-c2 vector with N-terminal or with N- as well as C-terminal flanking sequences, and expressed in fusion with maltose binding protein. The proteinase self-processed itself from the fusion protein during expression and formed inclusion bodies. The enzyme was purified from inclusion bodies by cation-exchange chromatography followed by gel filtration. Specificity of the enzyme was compared to that of human T-cell leukemia proteinase type 1. Although the two viruses belong to the same subfamily of retroviruses, the differences in their proteinase specificity, based on kinetics with oligopeptide substrates representing naturally occurring cleavage sites as well as on inhibition pattern, appear to be pronounced. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1478
Issue number1
DOIs
Publication statusPublished - Mar 16 2000

Fingerprint

Bovine Leukemia Virus
Deltaretrovirus
T-cells
Cloning
Viruses
Escherichia coli
Organism Cloning
Peptide Hydrolases
Inclusion Bodies
Fusion reactions
Maltose-Binding Proteins
T-Cell Leukemia
Oligopeptides
Retroviridae
Enzymes
Chromatography
Gel Chromatography
Cations
Ion exchange
Gels

Keywords

  • BLV proteinase
  • HTLV-1 proteinase
  • Inhibitor binding
  • Oligopeptide substrate
  • Substrate specificity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology
  • Biophysics

Cite this

Cloning of the bovine leukemia virus proteinase in Escherichia coli and comparison of its specificity to that of human T-cell leukemia virus proteinase. / Zahuczky, Gábor; Boross, Péter; Bagossi, P.; Emri, Gabriella; Copeland, Terry D.; Oroszlan, Stephen; Louis, John M.; Tőzsér, J.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 1478, No. 1, 16.03.2000, p. 1-8.

Research output: Contribution to journalArticle

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