Cloning of caf1+, caf2+ and caf4+ from Schizosaccharomyces pombe: Their involvement in multidrug resistance, UV and pH sensitivity

Z. Benko, M. Sipiczki, A. M. Carr

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We previously identified four nuclear genes (caf1+ to caf4+) in Schizosaccharomyces pombe, mutations in which can confer caffeine resistance. Here we report the cloning and sequencing of caf1+, caf2+ and caf4+. All three genes are allelic to genes (hba1+, crm1+ and trr1+, respectively) involved in multidrug resistance mechanisms or in stress response systems. In agreement with this the caffeine-resistant mutants caf1 (hba1)-21, caf2 (crm1)-3 and caf4 (trr1)-83 are also resistant to brefeldin. Disruption of caf1 (hba1)+ and caf4 (trr1)+ makes cells sensitive to high pH. The overlapping ranges of pleiotropic effects and the genetic interaction detected between caf1 (hba1)+ and caf2 (crm1)+ suggest that the three genes function in interlinked systems.

Original languageEnglish
Pages (from-to)434-443
Number of pages10
JournalMolecular and General Genetics
Volume260
Issue number5
DOIs
Publication statusPublished - 1998

Keywords

  • Brefeldin A resistance
  • Caffeine resistance
  • Cell cycle
  • Multidrug resistance
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Genetics

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