Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa)

A role in hormone-mediated cell division?

Heather I. McKhann, Florian Frugier, György Petrovics, Teodoro Coba De La Peña, Edouard Jurkevitch, Spencer Brown, É. Kondorosi, Adam Kondorosi, Martin Crespi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Rhizobium meliloti can interact symbiotically with Medicago plants thereby inducing the formation of root nodules. Screening of a young nodule cDNA library led to the isolation of a cDNA from Medicago sativa, Msgbl, that comprises a new member of the RACK1 (Receptor of Activated C Kinase) subfamily of WD-repeat proteins. This subfamily shows horology to the β-subunit of heterotrimeric G proteins. Besides RACK1, this subfamily contains several plant genes including the well characterized auxin-inducible ArcA of tobacco. The Msgbl gene is strongly expressed in young embryos and in leaves and is induced upon cytokinin treatment of roots. Whereas northern analysis failed to reveal differences in expression between total RNA from roots and nodules, in situ hybridization demonstrated that the transcript was most abundant in dividing cells of nodule primordia and in the nodule meristem. Msgbl may be related to the signal transduction acting in response to hormone-mediated cell division.

Original languageEnglish
Pages (from-to)771-780
Number of pages10
JournalPlant Molecular Biology
Volume34
Issue number5
DOIs
Publication statusPublished - Jul 1997

Fingerprint

Sinorhizobium meliloti
Heterotrimeric GTP-Binding Proteins
Cytokinins
Medicago
Plant Genes
Indoleacetic Acids
Meristem
Medicago sativa
Cloning
Gene Library
Cell Division
Tobacco
In Situ Hybridization
cell division
Organism Cloning
alfalfa
molecular cloning
Signal Transduction
Embryonic Structures
Complementary DNA

Keywords

  • Alfalfa
  • Cytokinin
  • G-protein β-subunit
  • RACK1 subfamily
  • WD-repeat proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

McKhann, H. I., Frugier, F., Petrovics, G., Coba De La Peña, T., Jurkevitch, E., Brown, S., ... Crespi, M. (1997). Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): A role in hormone-mediated cell division? Plant Molecular Biology, 34(5), 771-780. https://doi.org/10.1023/A:1005899410389

Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa) : A role in hormone-mediated cell division? / McKhann, Heather I.; Frugier, Florian; Petrovics, György; Coba De La Peña, Teodoro; Jurkevitch, Edouard; Brown, Spencer; Kondorosi, É.; Kondorosi, Adam; Crespi, Martin.

In: Plant Molecular Biology, Vol. 34, No. 5, 07.1997, p. 771-780.

Research output: Contribution to journalArticle

McKhann, HI, Frugier, F, Petrovics, G, Coba De La Peña, T, Jurkevitch, E, Brown, S, Kondorosi, É, Kondorosi, A & Crespi, M 1997, 'Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): A role in hormone-mediated cell division?', Plant Molecular Biology, vol. 34, no. 5, pp. 771-780. https://doi.org/10.1023/A:1005899410389
McKhann, Heather I. ; Frugier, Florian ; Petrovics, György ; Coba De La Peña, Teodoro ; Jurkevitch, Edouard ; Brown, Spencer ; Kondorosi, É. ; Kondorosi, Adam ; Crespi, Martin. / Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa) : A role in hormone-mediated cell division?. In: Plant Molecular Biology. 1997 ; Vol. 34, No. 5. pp. 771-780.
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