Cloning of a gene highly overexpressed in cancer coding for a novel KH-domain containing protein

Friederike Müeller-Pillasch, Ulrike Lacher, Christine Wallrapp, Anne Micha, Frank Zimmerhackl, Horst Hameister, Gabor Varga, Helmut Friess, Markus Büchler, Hans Günther Beger, Maya R. Vila, Guido Adler, Thomas M. Gress

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218 Citations (Scopus)

Abstract

In a previous large scale screen for differentially expressed genes in pancreatic cancer, we identified a gene highly overexpressed in cancer encoding a novel protein with four K-homologous (KH) domains. KH-domains are found in a subset of RNA-binding proteins, including pre-mRNA-binding (hnRNP) K protein and the fragile X mental retardation gene product (FMR1). By fluorescence in situ hybridization (FISH) the identified gene named koc (KH domain containing protein overexpressed in cancer) was assigned to chromosome 7p11.5. Two pseudogenes were localised on chromosome 6 and 11. The cloned hoc cDNA has a 250 bp 5'-UTR, a 1740 bp ORF and a 2168 bp 3'-UTR. The AU-rich 3'-untranslated region of hoc contains eight AUUUA and four AUUUUUA reiterated motifs. The deduced koc protein with 580 amino-acids has a relative molecular mass (M(r)) of approximately 65,000 (65 K). The koc transcript is highly overexpressed in pancreatic cancer cell lines and in pancreatic cancer tissue as compared to both, normal pancreas and chronic pancreatitis tissue. High levels of expression were as well found in tissue samples of other human tumours. As the KH domain has been shown to be involved in the regulation of RNA synthesis and metabolism, we speculate that hoc may assume a role in the regulation of tumour cell proliferation by interfering with transcriptional and or posttranscriptional processes. However, the precise role of koc in human tumour cells is unknown and remains to be elucidated.

Original languageEnglish
Pages (from-to)2729-2733
Number of pages5
JournalOncogene
Volume14
Issue number22
DOIs
Publication statusPublished - Jul 18 1997

Keywords

  • KH-domain
  • Overexpression
  • Pancreatic cancer
  • RNA-binding

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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  • Cite this

    Müeller-Pillasch, F., Lacher, U., Wallrapp, C., Micha, A., Zimmerhackl, F., Hameister, H., Varga, G., Friess, H., Büchler, M., Beger, H. G., Vila, M. R., Adler, G., & Gress, T. M. (1997). Cloning of a gene highly overexpressed in cancer coding for a novel KH-domain containing protein. Oncogene, 14(22), 2729-2733. https://doi.org/10.1038/sj.onc.1201110