Cloning, expression and purification of SmpB from Mycobacterium Tuberculosis

L. Kovács, Klára Megyeri, Anna Juhász, Anikó Zaja, A. Miczák

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Abstract

SmpB, a small tmRNA binding protein, is essential for trans-translation. 6His and FLAG tagged SmpB was cloned from Mycobacterium tuberculosis H37Rv. It was expressed in Escherichia coli using the T7 promoter-polymerase system. Anti-FLAG M2 agarose was used for its purification. Mycobacterial SmpB copurifies with other proteins. We identified elongation factor EF-Tu in the purified SmpB preparations.

Original languageEnglish
Pages (from-to)297-302
Number of pages6
JournalActa microbiologica et immunologica Hungarica
Volume51
Issue number3
DOIs
Publication statusPublished - Dec 1 2004

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Keywords

  • EF-Tu
  • M. tuberculosis
  • Smpb
  • tmRNA

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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