Cloning, chromosomal localization, and functional expression of the α1 subunit of the L-type voltage-dependent calcium channel from normal human heart

David Schultz, G. Mikala, Atsuko Yatani, Dorothy B. Engle, David E. Iles, Bart Segers, Richard J. Sinke, Daniël Olde Weghuis, Udo Klöckner, Minoru Wakamori, Jing Jing Wang, David Melvin, Gyula Varadi, Arnold Schwartz

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

A unique structural variant of the cardiac L-type voltage-dependent calcium channel α1 subunit cDNA was isolated from libraries derived from normal human heart mRNA. The deduced amino acid sequence shows significant homology to other calcium channel α1 subunits. However, differences from the rabbit heart α1 include a shortened N-terminus, a unique C-terminal insertion, and both forms of an alternatively spliced motif IV S3 region. The shortened N-terminus provides optimal access to consensus sequences thought to facilitate translation. Northern blot analysis revealed a single hybridizing mRNA species of 9.4 kb. The gene for the human heart α1 subunit was localized specifically to the distal region of chromosome 12p13. The cloned α1 subunit was expressed in Xenopus oocytes and single-channel analyses revealed native-like pharmacology and channel properties.

Original languageEnglish
Pages (from-to)6228-6232
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number13
Publication statusPublished - Jul 1 1993

Fingerprint

L-Type Calcium Channels
Organism Cloning
Messenger RNA
Consensus Sequence
Calcium Channels
Xenopus
Northern Blotting
Libraries
Oocytes
Amino Acid Sequence
Complementary DNA
Chromosomes
Pharmacology
Rabbits
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Cloning, chromosomal localization, and functional expression of the α1 subunit of the L-type voltage-dependent calcium channel from normal human heart. / Schultz, David; Mikala, G.; Yatani, Atsuko; Engle, Dorothy B.; Iles, David E.; Segers, Bart; Sinke, Richard J.; Weghuis, Daniël Olde; Klöckner, Udo; Wakamori, Minoru; Wang, Jing Jing; Melvin, David; Varadi, Gyula; Schwartz, Arnold.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 13, 01.07.1993, p. 6228-6232.

Research output: Contribution to journalArticle

Schultz, D, Mikala, G, Yatani, A, Engle, DB, Iles, DE, Segers, B, Sinke, RJ, Weghuis, DO, Klöckner, U, Wakamori, M, Wang, JJ, Melvin, D, Varadi, G & Schwartz, A 1993, 'Cloning, chromosomal localization, and functional expression of the α1 subunit of the L-type voltage-dependent calcium channel from normal human heart', Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 13, pp. 6228-6232.
Schultz, David ; Mikala, G. ; Yatani, Atsuko ; Engle, Dorothy B. ; Iles, David E. ; Segers, Bart ; Sinke, Richard J. ; Weghuis, Daniël Olde ; Klöckner, Udo ; Wakamori, Minoru ; Wang, Jing Jing ; Melvin, David ; Varadi, Gyula ; Schwartz, Arnold. / Cloning, chromosomal localization, and functional expression of the α1 subunit of the L-type voltage-dependent calcium channel from normal human heart. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 13. pp. 6228-6232.
@article{200f50fba3a8474eb96dc7502cd54f69,
title = "Cloning, chromosomal localization, and functional expression of the α1 subunit of the L-type voltage-dependent calcium channel from normal human heart",
abstract = "A unique structural variant of the cardiac L-type voltage-dependent calcium channel α1 subunit cDNA was isolated from libraries derived from normal human heart mRNA. The deduced amino acid sequence shows significant homology to other calcium channel α1 subunits. However, differences from the rabbit heart α1 include a shortened N-terminus, a unique C-terminal insertion, and both forms of an alternatively spliced motif IV S3 region. The shortened N-terminus provides optimal access to consensus sequences thought to facilitate translation. Northern blot analysis revealed a single hybridizing mRNA species of 9.4 kb. The gene for the human heart α1 subunit was localized specifically to the distal region of chromosome 12p13. The cloned α1 subunit was expressed in Xenopus oocytes and single-channel analyses revealed native-like pharmacology and channel properties.",
author = "David Schultz and G. Mikala and Atsuko Yatani and Engle, {Dorothy B.} and Iles, {David E.} and Bart Segers and Sinke, {Richard J.} and Weghuis, {Dani{\"e}l Olde} and Udo Kl{\"o}ckner and Minoru Wakamori and Wang, {Jing Jing} and David Melvin and Gyula Varadi and Arnold Schwartz",
year = "1993",
month = "7",
day = "1",
language = "English",
volume = "90",
pages = "6228--6232",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "13",

}

TY - JOUR

T1 - Cloning, chromosomal localization, and functional expression of the α1 subunit of the L-type voltage-dependent calcium channel from normal human heart

AU - Schultz, David

AU - Mikala, G.

AU - Yatani, Atsuko

AU - Engle, Dorothy B.

AU - Iles, David E.

AU - Segers, Bart

AU - Sinke, Richard J.

AU - Weghuis, Daniël Olde

AU - Klöckner, Udo

AU - Wakamori, Minoru

AU - Wang, Jing Jing

AU - Melvin, David

AU - Varadi, Gyula

AU - Schwartz, Arnold

PY - 1993/7/1

Y1 - 1993/7/1

N2 - A unique structural variant of the cardiac L-type voltage-dependent calcium channel α1 subunit cDNA was isolated from libraries derived from normal human heart mRNA. The deduced amino acid sequence shows significant homology to other calcium channel α1 subunits. However, differences from the rabbit heart α1 include a shortened N-terminus, a unique C-terminal insertion, and both forms of an alternatively spliced motif IV S3 region. The shortened N-terminus provides optimal access to consensus sequences thought to facilitate translation. Northern blot analysis revealed a single hybridizing mRNA species of 9.4 kb. The gene for the human heart α1 subunit was localized specifically to the distal region of chromosome 12p13. The cloned α1 subunit was expressed in Xenopus oocytes and single-channel analyses revealed native-like pharmacology and channel properties.

AB - A unique structural variant of the cardiac L-type voltage-dependent calcium channel α1 subunit cDNA was isolated from libraries derived from normal human heart mRNA. The deduced amino acid sequence shows significant homology to other calcium channel α1 subunits. However, differences from the rabbit heart α1 include a shortened N-terminus, a unique C-terminal insertion, and both forms of an alternatively spliced motif IV S3 region. The shortened N-terminus provides optimal access to consensus sequences thought to facilitate translation. Northern blot analysis revealed a single hybridizing mRNA species of 9.4 kb. The gene for the human heart α1 subunit was localized specifically to the distal region of chromosome 12p13. The cloned α1 subunit was expressed in Xenopus oocytes and single-channel analyses revealed native-like pharmacology and channel properties.

UR - http://www.scopus.com/inward/record.url?scp=0027162740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027162740&partnerID=8YFLogxK

M3 - Article

C2 - 8392192

AN - SCOPUS:0027162740

VL - 90

SP - 6228

EP - 6232

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 13

ER -