Cloning, characterization and localization of Chinese hamster HP1 isoforms.

Barnabás Szakál, Imre Cserpán, Erika Csonka, E. Monostori, A. Udvardy, Gyula Hadlaczky

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Chinese hamster is one of the few mammalian species that are characterized by relatively poor heterochromatin content. It was intriguing to test whether or not the lack of large blocks of heterochromatin in the hamster chromosomes could be correlated with the absence or species-specific differences of the HP1 proteins, the main structural components of heterochromatin. To address this, we attempted to clone HP1 from the Chinese hamster. It is shown here that all three isoforms of HP1 known in mammals are present in hamster, and the amino acid sequences deduced from the cDNAs of the isoforms are 97-100% identical to those of the known mammalian homologues. All three isoforms are localized mainly in heterochromatic regions in the native chromosomes and nuclei. The hamster HP1 alpha gene was cloned, sequenced and mapped to the short arm of hamster chromosome 2.These data indicate that the Chinese hamster has all the HP1 components necessary for the establishment of heterochromatin. The limited amount of heterochromatin in hamster cells may probably be attributed to the unusual satellite DNA content of the hamster genome.

Original languageEnglish
Pages (from-to)483-493
Number of pages11
JournalChromosome Research
Volume12
Issue number5
Publication statusPublished - 2004

Fingerprint

Cricetulus
Heterochromatin
Cricetinae
Organism Cloning
Protein Isoforms
Chromosomes
Satellite DNA
Chromosomes, Human, Pair 2
Mammals
Amino Acid Sequence
Complementary DNA
Clone Cells
Genome
Genes
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Szakál, B., Cserpán, I., Csonka, E., Monostori, E., Udvardy, A., & Hadlaczky, G. (2004). Cloning, characterization and localization of Chinese hamster HP1 isoforms. Chromosome Research, 12(5), 483-493.

Cloning, characterization and localization of Chinese hamster HP1 isoforms. / Szakál, Barnabás; Cserpán, Imre; Csonka, Erika; Monostori, E.; Udvardy, A.; Hadlaczky, Gyula.

In: Chromosome Research, Vol. 12, No. 5, 2004, p. 483-493.

Research output: Contribution to journalArticle

Szakál, B, Cserpán, I, Csonka, E, Monostori, E, Udvardy, A & Hadlaczky, G 2004, 'Cloning, characterization and localization of Chinese hamster HP1 isoforms.', Chromosome Research, vol. 12, no. 5, pp. 483-493.
Szakál, Barnabás ; Cserpán, Imre ; Csonka, Erika ; Monostori, E. ; Udvardy, A. ; Hadlaczky, Gyula. / Cloning, characterization and localization of Chinese hamster HP1 isoforms. In: Chromosome Research. 2004 ; Vol. 12, No. 5. pp. 483-493.
@article{164f67332ed5406ebce2868f4283fcc0,
title = "Cloning, characterization and localization of Chinese hamster HP1 isoforms.",
abstract = "The Chinese hamster is one of the few mammalian species that are characterized by relatively poor heterochromatin content. It was intriguing to test whether or not the lack of large blocks of heterochromatin in the hamster chromosomes could be correlated with the absence or species-specific differences of the HP1 proteins, the main structural components of heterochromatin. To address this, we attempted to clone HP1 from the Chinese hamster. It is shown here that all three isoforms of HP1 known in mammals are present in hamster, and the amino acid sequences deduced from the cDNAs of the isoforms are 97-100{\%} identical to those of the known mammalian homologues. All three isoforms are localized mainly in heterochromatic regions in the native chromosomes and nuclei. The hamster HP1 alpha gene was cloned, sequenced and mapped to the short arm of hamster chromosome 2.These data indicate that the Chinese hamster has all the HP1 components necessary for the establishment of heterochromatin. The limited amount of heterochromatin in hamster cells may probably be attributed to the unusual satellite DNA content of the hamster genome.",
author = "Barnab{\'a}s Szak{\'a}l and Imre Cserp{\'a}n and Erika Csonka and E. Monostori and A. Udvardy and Gyula Hadlaczky",
year = "2004",
language = "English",
volume = "12",
pages = "483--493",
journal = "Chromosome Research",
issn = "0967-3849",
publisher = "Springer Netherlands",
number = "5",

}

TY - JOUR

T1 - Cloning, characterization and localization of Chinese hamster HP1 isoforms.

AU - Szakál, Barnabás

AU - Cserpán, Imre

AU - Csonka, Erika

AU - Monostori, E.

AU - Udvardy, A.

AU - Hadlaczky, Gyula

PY - 2004

Y1 - 2004

N2 - The Chinese hamster is one of the few mammalian species that are characterized by relatively poor heterochromatin content. It was intriguing to test whether or not the lack of large blocks of heterochromatin in the hamster chromosomes could be correlated with the absence or species-specific differences of the HP1 proteins, the main structural components of heterochromatin. To address this, we attempted to clone HP1 from the Chinese hamster. It is shown here that all three isoforms of HP1 known in mammals are present in hamster, and the amino acid sequences deduced from the cDNAs of the isoforms are 97-100% identical to those of the known mammalian homologues. All three isoforms are localized mainly in heterochromatic regions in the native chromosomes and nuclei. The hamster HP1 alpha gene was cloned, sequenced and mapped to the short arm of hamster chromosome 2.These data indicate that the Chinese hamster has all the HP1 components necessary for the establishment of heterochromatin. The limited amount of heterochromatin in hamster cells may probably be attributed to the unusual satellite DNA content of the hamster genome.

AB - The Chinese hamster is one of the few mammalian species that are characterized by relatively poor heterochromatin content. It was intriguing to test whether or not the lack of large blocks of heterochromatin in the hamster chromosomes could be correlated with the absence or species-specific differences of the HP1 proteins, the main structural components of heterochromatin. To address this, we attempted to clone HP1 from the Chinese hamster. It is shown here that all three isoforms of HP1 known in mammals are present in hamster, and the amino acid sequences deduced from the cDNAs of the isoforms are 97-100% identical to those of the known mammalian homologues. All three isoforms are localized mainly in heterochromatic regions in the native chromosomes and nuclei. The hamster HP1 alpha gene was cloned, sequenced and mapped to the short arm of hamster chromosome 2.These data indicate that the Chinese hamster has all the HP1 components necessary for the establishment of heterochromatin. The limited amount of heterochromatin in hamster cells may probably be attributed to the unusual satellite DNA content of the hamster genome.

UR - http://www.scopus.com/inward/record.url?scp=16644379775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16644379775&partnerID=8YFLogxK

M3 - Article

VL - 12

SP - 483

EP - 493

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 5

ER -