Cloning and structure of the BepI modification methylase

Dagmar Kupper, Jian Guang Zhou, Pal Venetianer, A. Kiss

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The gene coding for a CGCG specific DNA methylase has been cloned in E. coli from Brevibacterium epidermidis. The enzyme, named BepI methylase, is probably the cognate methylase of the FnuDII isoschizomer BepI endonuclease isolated from this strain. The expression of BepI methylase in E. coli is dependent on the orientation of the cloned fragment suggesting that the gene is transcribed from a promoter on the plasmid vector. No BepI endonuclease could be detected in the clones producing BepI methylase. The nucleotide sequence of the BepI methylase gene has been determined, it predicts a protein of 403 amino acids (M: 45, 447). Analysis of the amino acid sequence deduced from the nucleotide sequence revealed similarities between the BepI methylase and other cytosine methylases. M.BepI methylates the external cytosine in its recognition sequence.

Original languageEnglish
Pages (from-to)1077-1088
Number of pages12
JournalNucleic Acids Research
Volume17
Issue number3
DOIs
Publication statusPublished - Feb 11 1989

Fingerprint

DNA Modification Methylases
Cloning
Organism Cloning
Genes
Nucleotides
Gene
Escherichia coli
Escherichia Coli
Amino acids
Amino Acid Sequence
Clone
Promoter
Cytosine
Amino Acids
Fragment
Enzymes
DNA
Coding
Proteins
Protein

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Cloning and structure of the BepI modification methylase. / Kupper, Dagmar; Zhou, Jian Guang; Venetianer, Pal; Kiss, A.

In: Nucleic Acids Research, Vol. 17, No. 3, 11.02.1989, p. 1077-1088.

Research output: Contribution to journalArticle

Kupper, Dagmar ; Zhou, Jian Guang ; Venetianer, Pal ; Kiss, A. / Cloning and structure of the BepI modification methylase. In: Nucleic Acids Research. 1989 ; Vol. 17, No. 3. pp. 1077-1088.
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