Cloning and sequence analysis of Mucor circinelloides glyceraldehyde-3-phosphate dehydrogenase gene

Klára Ács, Zs Kasza, Gyöngyi Lukács, H. Schwab, Cs Vágvölgyi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A genomic library of Mucor circinelloides ATCC 1216b has been constructed in Lambda Fix II vector. The library has an average insert site of 10 kb and covers the genome 12 times. The M. circinelloides gene encoding glyceraldehyde-3-phosphate dehydrogenase (gpd) was isolated from this library by hybridization of the recombinant phage clones with a gpd-specific gene probe generated by PCR reaction. The complete nucleotide sequence encodes a putative polypeptide chain of 339 amino acids interrupted by 3 introns. The predicted amino acid sequence of this gene shows a high degree of sequence similarity to the GPD proteins from other filamentous fungi. The promoter region, containing a consensus TATA and CAAT box and a 298 nucleotid long termination region were also determined.

Original languageEnglish
Pages (from-to)305-312
Number of pages8
JournalActa microbiologica et immunologica Hungarica
Volume49
Issue number2-3
DOIs
Publication statusPublished - Dec 1 2002

Keywords

  • Cloning of glyceraldehyde-3-phosphate dehydrogenase
  • Mucor circinelloides

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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