Cloning and sequence analysis of cDNA for rat liver uricase

K. Motojima, S. Kanaya, S. Goto

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have isolated cDNA clones for rat liver uricase using an oligonucleotide corresponding to the N-terminal sequence of 8 amino acids. The nucleotide sequences of the cDNAs have been determined, and the amino acid sequence of the protein deduced. A 867-base open reading frame coding for 289 amino acids, corresponding to a molecular mass of 33,274 daltons, was confirmed by matching eight sequences of a total of 53 amino acids from peptide sequence analyses of the fragments generated by lysyl endopeptidase digestion of purified rat liver uricase. The deduced amino acid sequence of rat liver uricase shares 40% homology with that of soybean nodulin-specific uricase and has an N-terminal extension of 7 amino acids. In contrast, soybean uricase has a C-terminal extension of 12 amino acids, which is presumably the result of local gene duplication. Completely different N- and C-terminal structures of the two uricases suggest that the signals for targeting the proteins to the peroxisome are not located on the terminal continuous stretches of amino acids.

Original languageEnglish
Pages (from-to)16677-16681
Number of pages5
JournalJournal of Biological Chemistry
Volume263
Issue number32
Publication statusPublished - 1988

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Urate Oxidase
Cloning
Liver
Sequence Analysis
Rats
Organism Cloning
Complementary DNA
Amino Acids
Amino Acid Sequence
lysyl endopeptidase
Protein Sequence Analysis
Soybeans
Gene Duplication
Peroxisomes
Protein Transport
Oligonucleotides
Open Reading Frames
Digestion
Clone Cells
Molecular mass

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cloning and sequence analysis of cDNA for rat liver uricase. / Motojima, K.; Kanaya, S.; Goto, S.

In: Journal of Biological Chemistry, Vol. 263, No. 32, 1988, p. 16677-16681.

Research output: Contribution to journalArticle

Motojima, K, Kanaya, S & Goto, S 1988, 'Cloning and sequence analysis of cDNA for rat liver uricase', Journal of Biological Chemistry, vol. 263, no. 32, pp. 16677-16681.
Motojima, K. ; Kanaya, S. ; Goto, S. / Cloning and sequence analysis of cDNA for rat liver uricase. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 32. pp. 16677-16681.
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