Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes

L. Szilák, Pál Venetianer, A. Kiss

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The genes coding for the GGNCC specific Sau96I estriction and modification enzymes were cloned and expressed in E. coli. The DNA sequence predicts a 430 amino acid protein (Mr: 49,252) for the methyltransferase and a 261 amino acid protein (Mr: 30,486) for the endonuclease. No protein sequence similarity was detected between the Sau96I methyltransferase and endonuclease. The methyltransferase contains the sequence elements characteristic for m5C-methyltransferases. In addition to this, M.Sau96I shows similarity, also in the variable region, with one m5C-methyltransferase (M.SinI) which has closely related recognition specificity (GGA/TCC). M.Sau96I methylates the internal cytosine within the GGNCC recognition sequence. The Sau96I endonuclease appears to act as a monomer.

Original languageEnglish
Pages (from-to)4659-4664
Number of pages6
JournalNucleic Acids Research
Volume18
Issue number16
Publication statusPublished - Aug 25 1990

Fingerprint

Cloning
Methyltransferases
Nucleotides
Amino Acids
Organism Cloning
Enzymes
Coding
Genes
Endonucleases
Restriction
Gene
Proteins
Protein
Amino acids
Protein Sequence
DNA Sequence
Escherichia Coli
Specificity
DNA sequences
Cytosine

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes. / Szilák, L.; Venetianer, Pál; Kiss, A.

In: Nucleic Acids Research, Vol. 18, No. 16, 25.08.1990, p. 4659-4664.

Research output: Contribution to journalArticle

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