Cloning and molecular characterization of a novel chromosome specific centromere sequence of Chinese hamster

K. Fátyol, Imre Cserpán, Tünde Praznovszky, Judit Kereso, Gyula Hadlaczky

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We isolated and characterized the first chromosome-specific satellite DNA (HC2sat) of Chinese hamster. This novel satellite was localized to the pericentric region of hamster chromosome 2. The 2.8 kb long repeat unit of HC2sat was identified and two such units were sequenced. Extended short range periodicity could not be revealed in repeat units. These elements are amongst the largest satellite repeat units reported from mammals to date. HC2sat is a major constituent of the pericentric region of CHO chromosome 2 representing a 7-14 Mb long DNA segment. Studies of long range organization of HC2sat indicated that the formation of the satellite array might occur in different phases and involved different amplification mechanisms.

Original languageEnglish
Pages (from-to)3728-3736
Number of pages9
JournalNucleic Acids Research
Volume22
Issue number18
Publication statusPublished - Sep 11 1994

Fingerprint

Chromosomes, Human, Pair 2
Centromere
Cloning
Molecular Cloning
Chromosomes
Cricetulus
Chromosome
Satellites
Satellite DNA
Unit
Periodicity
Cricetinae
Mammals
DNA
Amplification
Range of data
Hamster

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Cloning and molecular characterization of a novel chromosome specific centromere sequence of Chinese hamster. / Fátyol, K.; Cserpán, Imre; Praznovszky, Tünde; Kereso, Judit; Hadlaczky, Gyula.

In: Nucleic Acids Research, Vol. 22, No. 18, 11.09.1994, p. 3728-3736.

Research output: Contribution to journalArticle

Fátyol, K, Cserpán, I, Praznovszky, T, Kereso, J & Hadlaczky, G 1994, 'Cloning and molecular characterization of a novel chromosome specific centromere sequence of Chinese hamster', Nucleic Acids Research, vol. 22, no. 18, pp. 3728-3736.
Fátyol, K. ; Cserpán, Imre ; Praznovszky, Tünde ; Kereso, Judit ; Hadlaczky, Gyula. / Cloning and molecular characterization of a novel chromosome specific centromere sequence of Chinese hamster. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 18. pp. 3728-3736.
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