Cloning and functional analysis of tipE, a novel membrane protein that enhances drosophila para sodium channel function

Guoping Feng, P. Deák, Maninder Chopra, Linda M. Hall

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Voltage-dependent sodium channels are involved in the initiation and propagation of action potentials in many excitable cells. Here we report that tipE, a gene defined by a temperature-sensitive paralytic mutation in Drosophila, encodes a novel integral membrane protein that dramatically stimulates functional expression in Xenopus oocytes of the Drosophila sodium channel α subunit encoded by the paralytic (para) locus. Using a heat shock promoter to control tipE+ gene expression in transgenic flies, we demonstrate that tipE+ gene expression is required during pupal development to rescue adult paralysis. In addition, we demonstrate a role or the tipE gene product in adults.

Original languageEnglish
Pages (from-to)1001-1011
Number of pages11
JournalCell
Volume82
Issue number6
DOIs
Publication statusPublished - Sep 22 1995

Fingerprint

Functional analysis
Sodium Channels
Cloning
Gene expression
Drosophila
Organism Cloning
Membrane Proteins
Genes
Gene Expression
Xenopus
Diptera
Paralysis
Action Potentials
Oocytes
Shock
Hot Temperature
Mutation
Temperature
Electric potential

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Molecular Biology

Cite this

Cloning and functional analysis of tipE, a novel membrane protein that enhances drosophila para sodium channel function. / Feng, Guoping; Deák, P.; Chopra, Maninder; Hall, Linda M.

In: Cell, Vol. 82, No. 6, 22.09.1995, p. 1001-1011.

Research output: Contribution to journalArticle

Feng, Guoping ; Deák, P. ; Chopra, Maninder ; Hall, Linda M. / Cloning and functional analysis of tipE, a novel membrane protein that enhances drosophila para sodium channel function. In: Cell. 1995 ; Vol. 82, No. 6. pp. 1001-1011.
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