Cloning and expression of the chicken type 2 iodothyronine 5'- deiodinase

Balazs Gereben, Tibor Bartha, Helen M. Tu, John W. Harney, Peter Rudas, P. Reed Larsen

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The type 2 iodothyronine deiodinase (D2) is critical for the intracellular production of 3,5,3'-triiodothyronine from thyroxine. The D2 mRNA of higher vertebrates is over 6 kilobases (kb), and no complete cDNA clones have been reported. Using 5'- and 3'-rapid amplification of cDNA ends and two cDNA libraries, we have cloned the 6094-base pair full-length chicken D2 cDNA. The deduced protein is ~31 kDa and contains two inframe UGA codons presumably encoding selenocysteine. One of these is in the highly conserved active catalytic center; the other is near the carboxyl terminus. Unusual features of the cDNA include a selenocysteine insertion sequence element ~4.8 kb 3' to the UGA codon in the active center and three short open reading frames in the 5'-untranslated region. The K(m) of D2 is ~1.0 nM for thyroxine, and the reaction is insensitive to inhibition by 6-n- propylthiouracil. Chicken D2 is expressed as a single transcript of ~6 kb in different brain regions and in the thyroid and lung. Hypothyroidism increases D2 mRNA in the telencephalon. Unlike in mammals, D2 mRNA and activity are expressed in the liver of the chicken, suggesting a role for D2 in the generation of plasma 3,5,3'-triiodothyronine in this species.

Original languageEnglish
Pages (from-to)13768-13776
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number20
DOIs
Publication statusPublished - May 14 1999

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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