Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1α. High conservation between mammalian and insect sequences

V. Dombrádi, J. M. Axton, D. M. Glover, P. T W Cohen

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

A 1.2-kb clone containing the full coding sequence of a protein phosphatase 1 catalytic subunit has been isolated from a Drosophila head cDNA library. It encodes a polypeptide of 302 amino acids with a molecular mass of 34.5 kDa. The predicted protein sequence is 92% identical (94% similar) to rabbit protein phosphatase 1α (PP-1α) demonstrating strict conservation of the phosphatase catalytic subunit over a considerable evolutionary distance. Abundant 1.6-kb and 2.5-kb mRNA transcripts were detected throughout Drosophila development. The clone hybridised to four sites on Drosophila salivary gland polytene chromosomes. The major site is at 87B6-12 on the right arm of chromosome 3. In addition, there are three secondary sites, one on the same chromosome at 96A2-5 and two on the X chromosome at 9C1-2 and 13C1-2. Isolation of a further cDNA clone, hybridising to 9C1-2 and encoding part of the catalytic subunit 88% similar to Drosophila PP-1α, proves the existence of at least two transcriptionally active genes for protein phosphatase 1.

Original languageEnglish
Pages (from-to)603-610
Number of pages8
JournalEuropean Journal of Biochemistry
Volume183
Issue number3
Publication statusPublished - 1989

Fingerprint

Protein Phosphatase 1
Cloning
Chromosomes
Drosophila
Insects
Organism Cloning
Conservation
Complementary DNA
Clone Cells
Catalytic Domain
Polytene Chromosomes
Drosophila Proteins
Chromosomes, Human, Pair 3
Molecular mass
X Chromosome
Salivary Glands
Gene Library
Phosphoric Monoester Hydrolases
Proteins
Head

ASJC Scopus subject areas

  • Biochemistry

Cite this

@article{b6a9162cf356465d8d26e6130e45a555,
title = "Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1α. High conservation between mammalian and insect sequences",
abstract = "A 1.2-kb clone containing the full coding sequence of a protein phosphatase 1 catalytic subunit has been isolated from a Drosophila head cDNA library. It encodes a polypeptide of 302 amino acids with a molecular mass of 34.5 kDa. The predicted protein sequence is 92{\%} identical (94{\%} similar) to rabbit protein phosphatase 1α (PP-1α) demonstrating strict conservation of the phosphatase catalytic subunit over a considerable evolutionary distance. Abundant 1.6-kb and 2.5-kb mRNA transcripts were detected throughout Drosophila development. The clone hybridised to four sites on Drosophila salivary gland polytene chromosomes. The major site is at 87B6-12 on the right arm of chromosome 3. In addition, there are three secondary sites, one on the same chromosome at 96A2-5 and two on the X chromosome at 9C1-2 and 13C1-2. Isolation of a further cDNA clone, hybridising to 9C1-2 and encoding part of the catalytic subunit 88{\%} similar to Drosophila PP-1α, proves the existence of at least two transcriptionally active genes for protein phosphatase 1.",
author = "V. Dombr{\'a}di and Axton, {J. M.} and Glover, {D. M.} and Cohen, {P. T W}",
year = "1989",
language = "English",
volume = "183",
pages = "603--610",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1α. High conservation between mammalian and insect sequences

AU - Dombrádi, V.

AU - Axton, J. M.

AU - Glover, D. M.

AU - Cohen, P. T W

PY - 1989

Y1 - 1989

N2 - A 1.2-kb clone containing the full coding sequence of a protein phosphatase 1 catalytic subunit has been isolated from a Drosophila head cDNA library. It encodes a polypeptide of 302 amino acids with a molecular mass of 34.5 kDa. The predicted protein sequence is 92% identical (94% similar) to rabbit protein phosphatase 1α (PP-1α) demonstrating strict conservation of the phosphatase catalytic subunit over a considerable evolutionary distance. Abundant 1.6-kb and 2.5-kb mRNA transcripts were detected throughout Drosophila development. The clone hybridised to four sites on Drosophila salivary gland polytene chromosomes. The major site is at 87B6-12 on the right arm of chromosome 3. In addition, there are three secondary sites, one on the same chromosome at 96A2-5 and two on the X chromosome at 9C1-2 and 13C1-2. Isolation of a further cDNA clone, hybridising to 9C1-2 and encoding part of the catalytic subunit 88% similar to Drosophila PP-1α, proves the existence of at least two transcriptionally active genes for protein phosphatase 1.

AB - A 1.2-kb clone containing the full coding sequence of a protein phosphatase 1 catalytic subunit has been isolated from a Drosophila head cDNA library. It encodes a polypeptide of 302 amino acids with a molecular mass of 34.5 kDa. The predicted protein sequence is 92% identical (94% similar) to rabbit protein phosphatase 1α (PP-1α) demonstrating strict conservation of the phosphatase catalytic subunit over a considerable evolutionary distance. Abundant 1.6-kb and 2.5-kb mRNA transcripts were detected throughout Drosophila development. The clone hybridised to four sites on Drosophila salivary gland polytene chromosomes. The major site is at 87B6-12 on the right arm of chromosome 3. In addition, there are three secondary sites, one on the same chromosome at 96A2-5 and two on the X chromosome at 9C1-2 and 13C1-2. Isolation of a further cDNA clone, hybridising to 9C1-2 and encoding part of the catalytic subunit 88% similar to Drosophila PP-1α, proves the existence of at least two transcriptionally active genes for protein phosphatase 1.

UR - http://www.scopus.com/inward/record.url?scp=0024427481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024427481&partnerID=8YFLogxK

M3 - Article

VL - 183

SP - 603

EP - 610

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 3

ER -