Cloning and characterization of the dromedary (Camelus dromedarius) neonatal Fc receptor (drFcRn)

I. Kacskovics, B. Mayer, Zsuzsanna Kis, L. Frenyó, Yaofeng Zhao, Serge Muyldermans, Lennart Hammarström

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The full length cDNA of the dromedary neonatal Fc receptor (drFcRn) α chain was isolated and found that it is similar to the neonatal Fc receptor (FcRn) of other species with a high overall similarity to ruminant FcRn α chains. The drFcRn/Fc contact residues are highly conserved and predicted to bind both conventional (IgG1) and heavy chain (IgG2a, IgG3) antibodies. Using immunohistochemistry, we detected its expression in the hepatocytes and in epithelial cells of portal bile ductuli and also in the mammary gland acini and ducti. Remarkably, Ser313, that was identified to be crucial for apical to basolateral transcytosis, is substituted in the drFcRn α chain. The full length of the dog and orangutan FcRn α chains was also identified from databases. Analyzing the phylogenetic relatedness of this gene we found that dromedary clustered together with artiodactyls, dog is located between artiodactyls and primates, where the orangutan was branched, reflecting the accepted evolutionary relationships.

Original languageEnglish
Pages (from-to)1203-1215
Number of pages13
JournalDevelopmental and Comparative Immunology
Volume30
Issue number12
DOIs
Publication statusPublished - 2006

Fingerprint

Pongo
Camelus
Organism Cloning
Immunoglobulin G
Dogs
Transcytosis
Ruminants
Human Mammary Glands
Bile
Primates
Hepatocytes
Complementary DNA
Epithelial Cells
Immunohistochemistry
Databases
Antibodies
Genes
neonatal Fc receptors

Keywords

  • Antibodies
  • Dromedary
  • Fc receptors
  • Liver
  • Mammary gland

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Cloning and characterization of the dromedary (Camelus dromedarius) neonatal Fc receptor (drFcRn). / Kacskovics, I.; Mayer, B.; Kis, Zsuzsanna; Frenyó, L.; Zhao, Yaofeng; Muyldermans, Serge; Hammarström, Lennart.

In: Developmental and Comparative Immunology, Vol. 30, No. 12, 2006, p. 1203-1215.

Research output: Contribution to journalArticle

Kacskovics, I. ; Mayer, B. ; Kis, Zsuzsanna ; Frenyó, L. ; Zhao, Yaofeng ; Muyldermans, Serge ; Hammarström, Lennart. / Cloning and characterization of the dromedary (Camelus dromedarius) neonatal Fc receptor (drFcRn). In: Developmental and Comparative Immunology. 2006 ; Vol. 30, No. 12. pp. 1203-1215.
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