Cloning and characterization of Fpmtr1, an amino acid transporter gene of Fusarium proliferatum (Gibberella intermedia)

Apor Jeney, Emese Béki, Anita Keszthelyi, John F. Leslie, L. Hornok

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Fpmtr1, an amino acid transporter gene from Fusarium proliferatum was strongly expressed during conidial germination and repressed in late stationary phase. To identify the specific function of this gene, ΔFpmtr1 knock-out mutants were generated by gene replacement. Vegetative growth of the ΔFpmtr1 mutants was normal both in liquid and on solid media, but conidial germination was delayed. The ΔFpmtr1 mutants and the wild type were equally fertile when used as males in sexual crosses, however if the mutants were used as the female parent then the fertility of the cross decreased dramatically. Inactivation of Fpmtr1 abolished vegetative self-incompatibility in strain ITEM 2287 of F. proliferatum, but the ΔFpmtr1 mutants were still vegetatively incompatible with the other strains of the fungus. Endophytic colonization capability of the mutants, assessed on maize seedlings also was adversely affected. These data suggest that Fpmtr1 is involved in multiple developmental processes related to both sexual and parasexual events in F. proliferatum. Furthermore, the fungus might have problems in adapting to a less than optimal environment if this otherwise dispensable transporter has been inactivated.

Original languageEnglish
Pages (from-to)16-24
Number of pages9
JournalJournal of Basic Microbiology
Volume47
Issue number1
DOIs
Publication statusPublished - 2007

Fingerprint

Gibberella
Amino Acid Transport Systems
Fusarium
Germination
Organism Cloning
Fungi
Gene Knockout Techniques
Seedlings
Genes
Zea mays
Fertility
Growth

Keywords

  • Amino acid transporter
  • Expression
  • Fusarium

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Genetics

Cite this

Cloning and characterization of Fpmtr1, an amino acid transporter gene of Fusarium proliferatum (Gibberella intermedia). / Jeney, Apor; Béki, Emese; Keszthelyi, Anita; Leslie, John F.; Hornok, L.

In: Journal of Basic Microbiology, Vol. 47, No. 1, 2007, p. 16-24.

Research output: Contribution to journalArticle

Jeney, Apor ; Béki, Emese ; Keszthelyi, Anita ; Leslie, John F. ; Hornok, L. / Cloning and characterization of Fpmtr1, an amino acid transporter gene of Fusarium proliferatum (Gibberella intermedia). In: Journal of Basic Microbiology. 2007 ; Vol. 47, No. 1. pp. 16-24.
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