Clonality, expression and methylation patterns of the Epstein-Barr virus genomes in lethal midline granulomas classified as peripheral angiocentric T cell lymphomas

J. Mináróvits, L. F. Hu, S. Imai, Y. Harabuchi, A. Kataura, S. Minarovits-Kormuta, T. Osato, G. Klein

Research output: Contribution to journalArticle

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Abstract

We analysed the terminal repeats of Epstein-Barr virus (EBV) in DNAs isolated from six lethal midline granuloma (LMG) biopsies. A single fused terminal fragment could be detected in each case, indicating that these angiocentric peripheral T cell lymphomas represent clonal proliferations of cells infected with EBV on a single occasion. Using reverse transcriptase-PCR, we detected EBV nuclear antigen (EBNA) 1 and latent membrane protein (LMP) 1, but not EBNA 2 messages in LMG biopsy RNAs. The splicing pattern of the EBNA 1 message was consistent with the usage of a promoter localized in the BamHI F fragment (F promoter). The BamHI W fragment repeats and LMP-coding sequences were highly methylated in all cases. In contrast, the LMP regulatory sequences were found to be hypomethylated or partially methylated, as in LMP-expressing nasopharyngeal carcinomas.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalJournal of General Virology
Volume75
Issue number1
Publication statusPublished - 1994

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Lethal Midline Granuloma
Peripheral T-Cell Lymphoma
Epstein-Barr Virus Nuclear Antigens
Human Herpesvirus 4
Methylation
Membrane Proteins
Genome
Biopsy
Terminal Repeat Sequences
Reverse Transcriptase Polymerase Chain Reaction
Cell Proliferation
RNA
DNA

ASJC Scopus subject areas

  • Immunology
  • Virology

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Clonality, expression and methylation patterns of the Epstein-Barr virus genomes in lethal midline granulomas classified as peripheral angiocentric T cell lymphomas. / Mináróvits, J.; Hu, L. F.; Imai, S.; Harabuchi, Y.; Kataura, A.; Minarovits-Kormuta, S.; Osato, T.; Klein, G.

In: Journal of General Virology, Vol. 75, No. 1, 1994, p. 77-84.

Research output: Contribution to journalArticle

Mináróvits, J. ; Hu, L. F. ; Imai, S. ; Harabuchi, Y. ; Kataura, A. ; Minarovits-Kormuta, S. ; Osato, T. ; Klein, G. / Clonality, expression and methylation patterns of the Epstein-Barr virus genomes in lethal midline granulomas classified as peripheral angiocentric T cell lymphomas. In: Journal of General Virology. 1994 ; Vol. 75, No. 1. pp. 77-84.
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