Clinical and laboratory applications of slide based cytometry with the LSC, SFM and the iCYTE imaging cytometer instruments

Jozsef Bocsi, Ed Luther, Anja Mittag, Ingo Jensen, Ulrich Sack, Dominik Lenz, Lajos Trezl, V. Varga, B. Molnár, Attila Tarnok

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Background: Slide based cytometry (SBC) is a technology for the rapid stoichiometric analysis of cells fixed to surfaces. Its applications are highly versatile and ranges from the clinics to high throughput drug discovery. SBC is realized in different instruments such as the Laser Scanning Cytometer (LSC) and Scanning Fluorescent Microscope (SFM) and the novel inverted microscope based iCyte image cytometer (Compucyte Corp.). Methods: Fluorochrome labeled specimens were immobilized on microscopic slides. They were placed on a conventional fluorescence microscope and analyzed by photomultiplayers or digital camera. Data comparable to flow cytometry were generated. In addition, each individual event could be visualized. Applications: The major advantage of instruments is the combination of two features: a) the minimal sample volume needed, and b) the connection of fluorescence data and morphological information. Rare cells were detected, frequency of apoptosis by myricetin formaldehyde and H 2O 2 mixtures was determined;. Conclusion: LSC, SFM and the novel iCyte have a wide spectrum of applicability in SBC and can be introduced as a standard technology for multiple settings. In addition, the iCyte and SFM instrument is suited for high throughput screening by automation and may be in future adapted to telepathology due to their high quality images. (This study was supported by the IZKF-Leipzig, Germany and T 034245 OTKA, Hungary).

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD.V. Nicolau, R. Raghavachari
Pages30-40
Number of pages11
Volume5328
DOIs
Publication statusPublished - 2004
EventMicroarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II - San Jose, CA, United States
Duration: Jan 25 2004Jan 26 2004

Other

OtherMicroarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II
CountryUnited States
CitySan Jose, CA
Period1/25/041/26/04

Fingerprint

cytometry
chutes
Microscopes
microscopes
Scanning
Imaging techniques
scanning
Lasers
lasers
Fluorescence
Throughput
Hungary
fluorescence
Flow cytometry
digital cameras
apoptosis
Digital cameras
Cell death
automation
formaldehyde

Keywords

  • Apoptosis
  • Digital slide
  • Formaldehyde
  • Image cytometry
  • Laser scanning cytometry
  • Polyphenols
  • Scanning fluorescence microscopy

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Bocsi, J., Luther, E., Mittag, A., Jensen, I., Sack, U., Lenz, D., ... Tarnok, A. (2004). Clinical and laboratory applications of slide based cytometry with the LSC, SFM and the iCYTE imaging cytometer instruments. In D. V. Nicolau, & R. Raghavachari (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5328, pp. 30-40) https://doi.org/10.1117/12.529314

Clinical and laboratory applications of slide based cytometry with the LSC, SFM and the iCYTE imaging cytometer instruments. / Bocsi, Jozsef; Luther, Ed; Mittag, Anja; Jensen, Ingo; Sack, Ulrich; Lenz, Dominik; Trezl, Lajos; Varga, V.; Molnár, B.; Tarnok, Attila.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / D.V. Nicolau; R. Raghavachari. Vol. 5328 2004. p. 30-40.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bocsi, J, Luther, E, Mittag, A, Jensen, I, Sack, U, Lenz, D, Trezl, L, Varga, V, Molnár, B & Tarnok, A 2004, Clinical and laboratory applications of slide based cytometry with the LSC, SFM and the iCYTE imaging cytometer instruments. in DV Nicolau & R Raghavachari (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 5328, pp. 30-40, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II, San Jose, CA, United States, 1/25/04. https://doi.org/10.1117/12.529314
Bocsi J, Luther E, Mittag A, Jensen I, Sack U, Lenz D et al. Clinical and laboratory applications of slide based cytometry with the LSC, SFM and the iCYTE imaging cytometer instruments. In Nicolau DV, Raghavachari R, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 5328. 2004. p. 30-40 https://doi.org/10.1117/12.529314
Bocsi, Jozsef ; Luther, Ed ; Mittag, Anja ; Jensen, Ingo ; Sack, Ulrich ; Lenz, Dominik ; Trezl, Lajos ; Varga, V. ; Molnár, B. ; Tarnok, Attila. / Clinical and laboratory applications of slide based cytometry with the LSC, SFM and the iCYTE imaging cytometer instruments. Proceedings of SPIE - The International Society for Optical Engineering. editor / D.V. Nicolau ; R. Raghavachari. Vol. 5328 2004. pp. 30-40
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