Cleavage and sequence recognition of 2,6-diaminopurine-containing DNA by site-specific endonucleases

M. Szekeres, Andrey V. Matveyev

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The susceptibility of 2,6-diaminopurine (DAP)-containing bacteriophage DNA to several restriction and other endonucleases was examined. With the only exception of TaqI, these enzymes did not accept the modified base as a substitute for adenine. The phage DNA was extensively fragmented by the restriction endonucleases which recognize only G and C-containing sites. 5′-terminal analysis of the MspI and SmaI fragments revealed that d(DAP-T) basepairs can be mistaken by some enzymes for d(G-C) pairs.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalFEBS Letters
Volume222
Issue number1
DOIs
Publication statusPublished - Sep 28 1987

Fingerprint

Bacteriophages
Endonucleases
DNA Restriction Enzymes
Dilatation and Curettage
DNA
Adenine
Enzymes
2,6-diaminopurine

Keywords

  • (Bacteriophage)
  • DNA
  • Modified nucleotide
  • Restriction endonuclease
  • Sequence recognition

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cleavage and sequence recognition of 2,6-diaminopurine-containing DNA by site-specific endonucleases. / Szekeres, M.; Matveyev, Andrey V.

In: FEBS Letters, Vol. 222, No. 1, 28.09.1987, p. 89-94.

Research output: Contribution to journalArticle

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