Claudin-1 as a Biomarker of Cervical Cytology and Histology

Márta Benczik, Ádám Galamb, Róbert Koiss, Attila Kovács, Balázs Járay, Tamás Székely, Tímea Szekerczés, Z. Schaff, G. Sobel, Csaba Jeney

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Several immunochemistry tests are used for triaging human papilloma virus (HPV) and cytology positive cases in cervical cancer screening and as an adjunct test to diagnose cervical cancer. Claudin-1 (CLDN1) protein is a major component of the tight junction, shown to have altered expression in cervical cancer. In this study, value of CLDN1 was analysed as a screening and triage immunochemistry test compared to cytology and HPV testing. A population of 352 women attending colposcopic referral visits resulting in cervical conisation and a second population of 150 women attending routine gynaecological visits with negative cervical cytology were enrolled in a multi-centre clinical study in Hungary. Cytology and HPV (Genoid Full Spectrum HPV Amplification and Detection System) testing were carried out along with immunocytochemistry for CLDN1, and as a reference, using CINtec p16 Cytology Kit. Three different evaluation protocols were used which assessed immunostaining characteristics with or without cytological readings. High correlation observable between p16INK4a and CLDN1 established CLDN1 as a competing marker in cervical cancer. Concordance of CLDN1 immunostaining of cervical intraepithelial neoplasia 2 and above (CIN2+) positives was 84.0 % (73.8–89.3); concordance of CIN2+ negatives was 69.0 % (59.6–75.8). In conclusion, CLDN1 has similar diagnostic potential as p16INK4a, our results established it as a histological and cytological biomarker with the potential to improve the clinical performance of cervical cytology and histology.

Original languageEnglish
Pages (from-to)179-188
Number of pages10
JournalPathology and Oncology Research
Volume22
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Claudin-1
Papillomaviridae
Cell Biology
Histology
Biomarkers
Uterine Cervical Neoplasms
Immunochemistry
Claudins
Conization
Cervical Intraepithelial Neoplasia
Hungary
Tight Junctions
Triage
Early Detection of Cancer
Population
Reading
Referral and Consultation
Immunohistochemistry

Keywords

  • Biomarker
  • Cervical cancer
  • Claudin-1
  • Immunocytochemistry

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Benczik, M., Galamb, Á., Koiss, R., Kovács, A., Járay, B., Székely, T., ... Jeney, C. (2016). Claudin-1 as a Biomarker of Cervical Cytology and Histology. Pathology and Oncology Research, 22(1), 179-188. https://doi.org/10.1007/s12253-015-9990-z

Claudin-1 as a Biomarker of Cervical Cytology and Histology. / Benczik, Márta; Galamb, Ádám; Koiss, Róbert; Kovács, Attila; Járay, Balázs; Székely, Tamás; Szekerczés, Tímea; Schaff, Z.; Sobel, G.; Jeney, Csaba.

In: Pathology and Oncology Research, Vol. 22, No. 1, 01.01.2016, p. 179-188.

Research output: Contribution to journalArticle

Benczik, M, Galamb, Á, Koiss, R, Kovács, A, Járay, B, Székely, T, Szekerczés, T, Schaff, Z, Sobel, G & Jeney, C 2016, 'Claudin-1 as a Biomarker of Cervical Cytology and Histology', Pathology and Oncology Research, vol. 22, no. 1, pp. 179-188. https://doi.org/10.1007/s12253-015-9990-z
Benczik M, Galamb Á, Koiss R, Kovács A, Járay B, Székely T et al. Claudin-1 as a Biomarker of Cervical Cytology and Histology. Pathology and Oncology Research. 2016 Jan 1;22(1):179-188. https://doi.org/10.1007/s12253-015-9990-z
Benczik, Márta ; Galamb, Ádám ; Koiss, Róbert ; Kovács, Attila ; Járay, Balázs ; Székely, Tamás ; Szekerczés, Tímea ; Schaff, Z. ; Sobel, G. ; Jeney, Csaba. / Claudin-1 as a Biomarker of Cervical Cytology and Histology. In: Pathology and Oncology Research. 2016 ; Vol. 22, No. 1. pp. 179-188.
@article{d72408d8d1b94e528c371a290f4f9e26,
title = "Claudin-1 as a Biomarker of Cervical Cytology and Histology",
abstract = "Several immunochemistry tests are used for triaging human papilloma virus (HPV) and cytology positive cases in cervical cancer screening and as an adjunct test to diagnose cervical cancer. Claudin-1 (CLDN1) protein is a major component of the tight junction, shown to have altered expression in cervical cancer. In this study, value of CLDN1 was analysed as a screening and triage immunochemistry test compared to cytology and HPV testing. A population of 352 women attending colposcopic referral visits resulting in cervical conisation and a second population of 150 women attending routine gynaecological visits with negative cervical cytology were enrolled in a multi-centre clinical study in Hungary. Cytology and HPV (Genoid Full Spectrum HPV Amplification and Detection System) testing were carried out along with immunocytochemistry for CLDN1, and as a reference, using CINtec p16 Cytology Kit. Three different evaluation protocols were used which assessed immunostaining characteristics with or without cytological readings. High correlation observable between p16INK4a and CLDN1 established CLDN1 as a competing marker in cervical cancer. Concordance of CLDN1 immunostaining of cervical intraepithelial neoplasia 2 and above (CIN2+) positives was 84.0 {\%} (73.8–89.3); concordance of CIN2+ negatives was 69.0 {\%} (59.6–75.8). In conclusion, CLDN1 has similar diagnostic potential as p16INK4a, our results established it as a histological and cytological biomarker with the potential to improve the clinical performance of cervical cytology and histology.",
keywords = "Biomarker, Cervical cancer, Claudin-1, Immunocytochemistry",
author = "M{\'a}rta Benczik and {\'A}d{\'a}m Galamb and R{\'o}bert Koiss and Attila Kov{\'a}cs and Bal{\'a}zs J{\'a}ray and Tam{\'a}s Sz{\'e}kely and T{\'i}mea Szekercz{\'e}s and Z. Schaff and G. Sobel and Csaba Jeney",
year = "2016",
month = "1",
day = "1",
doi = "10.1007/s12253-015-9990-z",
language = "English",
volume = "22",
pages = "179--188",
journal = "Pathology and Oncology Research",
issn = "1219-4956",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Claudin-1 as a Biomarker of Cervical Cytology and Histology

AU - Benczik, Márta

AU - Galamb, Ádám

AU - Koiss, Róbert

AU - Kovács, Attila

AU - Járay, Balázs

AU - Székely, Tamás

AU - Szekerczés, Tímea

AU - Schaff, Z.

AU - Sobel, G.

AU - Jeney, Csaba

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Several immunochemistry tests are used for triaging human papilloma virus (HPV) and cytology positive cases in cervical cancer screening and as an adjunct test to diagnose cervical cancer. Claudin-1 (CLDN1) protein is a major component of the tight junction, shown to have altered expression in cervical cancer. In this study, value of CLDN1 was analysed as a screening and triage immunochemistry test compared to cytology and HPV testing. A population of 352 women attending colposcopic referral visits resulting in cervical conisation and a second population of 150 women attending routine gynaecological visits with negative cervical cytology were enrolled in a multi-centre clinical study in Hungary. Cytology and HPV (Genoid Full Spectrum HPV Amplification and Detection System) testing were carried out along with immunocytochemistry for CLDN1, and as a reference, using CINtec p16 Cytology Kit. Three different evaluation protocols were used which assessed immunostaining characteristics with or without cytological readings. High correlation observable between p16INK4a and CLDN1 established CLDN1 as a competing marker in cervical cancer. Concordance of CLDN1 immunostaining of cervical intraepithelial neoplasia 2 and above (CIN2+) positives was 84.0 % (73.8–89.3); concordance of CIN2+ negatives was 69.0 % (59.6–75.8). In conclusion, CLDN1 has similar diagnostic potential as p16INK4a, our results established it as a histological and cytological biomarker with the potential to improve the clinical performance of cervical cytology and histology.

AB - Several immunochemistry tests are used for triaging human papilloma virus (HPV) and cytology positive cases in cervical cancer screening and as an adjunct test to diagnose cervical cancer. Claudin-1 (CLDN1) protein is a major component of the tight junction, shown to have altered expression in cervical cancer. In this study, value of CLDN1 was analysed as a screening and triage immunochemistry test compared to cytology and HPV testing. A population of 352 women attending colposcopic referral visits resulting in cervical conisation and a second population of 150 women attending routine gynaecological visits with negative cervical cytology were enrolled in a multi-centre clinical study in Hungary. Cytology and HPV (Genoid Full Spectrum HPV Amplification and Detection System) testing were carried out along with immunocytochemistry for CLDN1, and as a reference, using CINtec p16 Cytology Kit. Three different evaluation protocols were used which assessed immunostaining characteristics with or without cytological readings. High correlation observable between p16INK4a and CLDN1 established CLDN1 as a competing marker in cervical cancer. Concordance of CLDN1 immunostaining of cervical intraepithelial neoplasia 2 and above (CIN2+) positives was 84.0 % (73.8–89.3); concordance of CIN2+ negatives was 69.0 % (59.6–75.8). In conclusion, CLDN1 has similar diagnostic potential as p16INK4a, our results established it as a histological and cytological biomarker with the potential to improve the clinical performance of cervical cytology and histology.

KW - Biomarker

KW - Cervical cancer

KW - Claudin-1

KW - Immunocytochemistry

UR - http://www.scopus.com/inward/record.url?scp=84951574284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951574284&partnerID=8YFLogxK

U2 - 10.1007/s12253-015-9990-z

DO - 10.1007/s12253-015-9990-z

M3 - Article

VL - 22

SP - 179

EP - 188

JO - Pathology and Oncology Research

JF - Pathology and Oncology Research

SN - 1219-4956

IS - 1

ER -