Clathrin polymerization is not required for bulk-phase endocytosis in rat fetal fibroblasts

Philippe Cupers, Alex Veithen, A. Kiss, Pierre Baudhuin, Pierre J. Courtoy

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

To assess the role of clathrin in the bulk endocytic flow of rat foetal fibroblasts, the rate of internalization of fluid-phase and membrane-lipid tracers were compared, under control conditions and after inhibition of endocytic clathrin-coated pit formation. After intracellular potassium depletion or upon cell transfer into 0.35 M NaCl, the rate of internalization of receptor-bound transferrin and the residual membrane area of plasmalemmal clathrin-coated pits and vesicles were similarly decreased by ~90%. In contrast, the initial rate (

Original languageEnglish
Pages (from-to)725-735
Number of pages11
JournalJournal of Cell Biology
Volume127
Issue number3
DOIs
Publication statusPublished - 1994

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Clathrin
Endocytosis
Polymerization
Fibroblasts
Clathrin-Coated Vesicles
Transferrin Receptors
Membrane Lipids
Potassium
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Clathrin polymerization is not required for bulk-phase endocytosis in rat fetal fibroblasts. / Cupers, Philippe; Veithen, Alex; Kiss, A.; Baudhuin, Pierre; Courtoy, Pierre J.

In: Journal of Cell Biology, Vol. 127, No. 3, 1994, p. 725-735.

Research output: Contribution to journalArticle

Cupers, Philippe ; Veithen, Alex ; Kiss, A. ; Baudhuin, Pierre ; Courtoy, Pierre J. / Clathrin polymerization is not required for bulk-phase endocytosis in rat fetal fibroblasts. In: Journal of Cell Biology. 1994 ; Vol. 127, No. 3. pp. 725-735.
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