Classification of reversed-phase columns based on their selectivity towards vancomycin compounds

Erik Haghedooren, José Diana, Béla Noszál, Jos Hoogmartens, Erwin Adams

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The selection of a reversed-phase liquid chromatographic (RP-LC) column with suitable selectivity for a particular separation is difficult if the brand name of the column is not known. The monographs of the European Pharmacopoeia and other official compendia for drug analysis only give a general description of the stationary phase to be used in the operating procedure of a liquid chromatographic method. A project to develop a chromatographic test procedure to characterise RP-LC C18 columns was started earlier and resulted in a fast, simple, repeatable and reproducible test procedure. Four column parameters, determined on 69 RP-LC C18 columns, allowed the characterisation and ranking of these columns. In this paper, an overview of this column classification system is given with an application on the separation of vancomycin and some of its impurities. It is shown that the column ranking system is a helpful tool in the selection of a suitable column.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalTalanta
Volume71
Issue number1
DOIs
Publication statusPublished - Jan 15 2007

Keywords

  • Chromatographic tests
  • Column characterisation
  • Column classification
  • Reversed-phase liquid chromatography
  • Vancomycin

ASJC Scopus subject areas

  • Analytical Chemistry

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