Classification and prediction of survival in patients with the leukemic phase of cutaneous T cell lymphoma

Laszlo Kari, Andrey Loboda, Michael Nebozhyn, Alain H. Rook, Eric C. Vonderheid, Calen Nichols, Dezso Virok, Celia Chang, Wen Hwai Horng, James Johnston, Maria Wysocka, Michael K. Showe, Louise C. Showe

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152 Citations (Scopus)

Abstract

We have used cDNA arrays to investigate gene expression patterns in peripheral blood mononuclear cells from patients with leukemic forms of cutaneous T cell lymphoma, primarily Sezary syndrome (SS). When expression data for patients with high blood tumor burden (Sezary cells >60% of the lymphocytes) and healthy controls are compared by Student's t test, at P > 0.01, we find 385 genes to be differentially expressed. Highly overexpressed genes include Th2 cells-specific transcription factors Gata-3 and Jun B, as well as integrin β1, proteoglycan 2, the RhoB oncogene, and dual specificity phosphatase 1. Highly underexpressed genes include CD26, Stat-4, and the IL-1 receptors. Message for plastin-T, not normally expressed in lymphoid tissue, is detected only in patient samples and may provide a new marker for diagnosis. Using penalized discriminant analysis, we have identified a panel of eight genes that can distinguish SS in patients with as few as 5% circulating tumor cells. This suggests that, even in early disease, Sezary cells produce chemokines and cytokines that induce an expression profile in the peripheral blood distinctive to SS. Finally, we show that using 10 genes, we can identify a class of patients who will succumb within six months of sampling regardless of their tumor burden.

Original languageEnglish
Pages (from-to)1477-1488
Number of pages12
JournalJournal of Experimental Medicine
Volume197
Issue number11
DOIs
Publication statusPublished - Jun 2 2003

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Keywords

  • CTCL
  • Class prediction
  • Discriminant analysis
  • Prognosis
  • cDNA microarrays

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Kari, L., Loboda, A., Nebozhyn, M., Rook, A. H., Vonderheid, E. C., Nichols, C., Virok, D., Chang, C., Horng, W. H., Johnston, J., Wysocka, M., Showe, M. K., & Showe, L. C. (2003). Classification and prediction of survival in patients with the leukemic phase of cutaneous T cell lymphoma. Journal of Experimental Medicine, 197(11), 1477-1488. https://doi.org/10.1084/jem.20021726