Classical and atypical neuroblastoma - Case reports

Lászlõ Csáthy, J. Kappelmayer, István Szegedi, Béla Kajtár, C. Kiss, Zsuzsanna Hevessy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Neuroblastoma is the most common extracranial solid tumor in early childhood with a pluripotent neural cell origin. The disease often infiltrates the bone marrow, and these cases are candidates for flow cytometric diagnosis and disease follow-up, more so in atypical cases that are diagnostically challenging by other methods. Here, we report two neuroblastoma cases, one with characteristic neuroblastoma cells forming Homer-Wright rosettes found in the bone marrow sample by histological examination. This case showed the classical immunophenotype with CD81/CD56/CD117 positive and CD45 negative labeling and 80% bone marrow infiltration. Minimal residual disease measurement was performed in a regular fashion, and flow cytometry results showed good correlation with other disease markers. Analyzing 300,000 events provided a sensitivity level of 0.01%. The second case showed atypical cell morphology on histological examination, after which flow cytometric analysis was initiated. Atypical cells displayed CD81 and bright CD56 but CD117 negative immunophenotype, with a 28% bone marrow infiltration. In most cases the primary tumor can be identified, but in this particular case only bone marrow involvement could be detected using flow cytometry, as such making it a powerful tool for diagnosis and disease monitoring.

Original languageEnglish
Pages (from-to)134-136
Number of pages3
JournalCytometry Part B - Clinical Cytometry
Volume80 B
Issue number2
DOIs
Publication statusPublished - Mar 2011

Fingerprint

Neuroblastoma
Bone Marrow
Flow Cytometry
Residual Neoplasm
Neoplasms

Keywords

  • bone marrow analysis
  • flow cytometry
  • neuroblastoma

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

Cite this

Classical and atypical neuroblastoma - Case reports. / Csáthy, Lászlõ; Kappelmayer, J.; Szegedi, István; Kajtár, Béla; Kiss, C.; Hevessy, Zsuzsanna.

In: Cytometry Part B - Clinical Cytometry, Vol. 80 B, No. 2, 03.2011, p. 134-136.

Research output: Contribution to journalArticle

Csáthy, Lászlõ ; Kappelmayer, J. ; Szegedi, István ; Kajtár, Béla ; Kiss, C. ; Hevessy, Zsuzsanna. / Classical and atypical neuroblastoma - Case reports. In: Cytometry Part B - Clinical Cytometry. 2011 ; Vol. 80 B, No. 2. pp. 134-136.
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