Classes and subclasses of rat antibodies

Reaction with the antigen and interaction of the complex with the complement system

G. A. Medgyesi, K. Miklos, J. Kulics, G. Füst, J. Gergely, H. Bazin

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Antibodies against the dinitroaminophenyl (DNAP) haptenic group were raised in outbred CFY rats using HSA or LPS as carrier. Antibodies isolated by immunoadsorbent techniques were resolved into fractions representing distinct isotypes, and the resulting fractions were tested for avidity. Subclass IgG2a was found to contain antibodies of an avidity index lower than those of other IgG subclasses or IgM. IgG2a was the only isotype detected when DNAP-LPS was used for immunization. Complexes containing defined isotypes were compared for their capacity to activate homologous complement. IgG1 type antibody-containing complexes displayed a low complement activating capacity compared with those containing IgG2b, IgG2c or IgG2a. The latter subclass when complexed with antigen can thus induce complement-dependent processes in spite of a low avidity. Insoluble complexes of IgG antibodies were rapidly solubilized in rat serum (CRA phenomenon), except those containing predominantly IgG2c.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalImmunology
Volume43
Issue number1
Publication statusPublished - 1981

Fingerprint

Antigen-Antibody Reactions
Antibodies
Immunoglobulin G
Immunosorbent Techniques
Antibody Affinity
Immunoglobulin M
Immunization
Antigens
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Classes and subclasses of rat antibodies : Reaction with the antigen and interaction of the complex with the complement system. / Medgyesi, G. A.; Miklos, K.; Kulics, J.; Füst, G.; Gergely, J.; Bazin, H.

In: Immunology, Vol. 43, No. 1, 1981, p. 171-176.

Research output: Contribution to journalArticle

@article{600cd86699f948c8994cdf7b5bf469f4,
title = "Classes and subclasses of rat antibodies: Reaction with the antigen and interaction of the complex with the complement system",
abstract = "Antibodies against the dinitroaminophenyl (DNAP) haptenic group were raised in outbred CFY rats using HSA or LPS as carrier. Antibodies isolated by immunoadsorbent techniques were resolved into fractions representing distinct isotypes, and the resulting fractions were tested for avidity. Subclass IgG2a was found to contain antibodies of an avidity index lower than those of other IgG subclasses or IgM. IgG2a was the only isotype detected when DNAP-LPS was used for immunization. Complexes containing defined isotypes were compared for their capacity to activate homologous complement. IgG1 type antibody-containing complexes displayed a low complement activating capacity compared with those containing IgG2b, IgG2c or IgG2a. The latter subclass when complexed with antigen can thus induce complement-dependent processes in spite of a low avidity. Insoluble complexes of IgG antibodies were rapidly solubilized in rat serum (CRA phenomenon), except those containing predominantly IgG2c.",
author = "Medgyesi, {G. A.} and K. Miklos and J. Kulics and G. F{\"u}st and J. Gergely and H. Bazin",
year = "1981",
language = "English",
volume = "43",
pages = "171--176",
journal = "Immunology",
issn = "0019-2805",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Classes and subclasses of rat antibodies

T2 - Reaction with the antigen and interaction of the complex with the complement system

AU - Medgyesi, G. A.

AU - Miklos, K.

AU - Kulics, J.

AU - Füst, G.

AU - Gergely, J.

AU - Bazin, H.

PY - 1981

Y1 - 1981

N2 - Antibodies against the dinitroaminophenyl (DNAP) haptenic group were raised in outbred CFY rats using HSA or LPS as carrier. Antibodies isolated by immunoadsorbent techniques were resolved into fractions representing distinct isotypes, and the resulting fractions were tested for avidity. Subclass IgG2a was found to contain antibodies of an avidity index lower than those of other IgG subclasses or IgM. IgG2a was the only isotype detected when DNAP-LPS was used for immunization. Complexes containing defined isotypes were compared for their capacity to activate homologous complement. IgG1 type antibody-containing complexes displayed a low complement activating capacity compared with those containing IgG2b, IgG2c or IgG2a. The latter subclass when complexed with antigen can thus induce complement-dependent processes in spite of a low avidity. Insoluble complexes of IgG antibodies were rapidly solubilized in rat serum (CRA phenomenon), except those containing predominantly IgG2c.

AB - Antibodies against the dinitroaminophenyl (DNAP) haptenic group were raised in outbred CFY rats using HSA or LPS as carrier. Antibodies isolated by immunoadsorbent techniques were resolved into fractions representing distinct isotypes, and the resulting fractions were tested for avidity. Subclass IgG2a was found to contain antibodies of an avidity index lower than those of other IgG subclasses or IgM. IgG2a was the only isotype detected when DNAP-LPS was used for immunization. Complexes containing defined isotypes were compared for their capacity to activate homologous complement. IgG1 type antibody-containing complexes displayed a low complement activating capacity compared with those containing IgG2b, IgG2c or IgG2a. The latter subclass when complexed with antigen can thus induce complement-dependent processes in spite of a low avidity. Insoluble complexes of IgG antibodies were rapidly solubilized in rat serum (CRA phenomenon), except those containing predominantly IgG2c.

UR - http://www.scopus.com/inward/record.url?scp=0019479885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019479885&partnerID=8YFLogxK

M3 - Article

VL - 43

SP - 171

EP - 176

JO - Immunology

JF - Immunology

SN - 0019-2805

IS - 1

ER -