CLASS FRACTIONATION OF ACIDIC GLYCOLIPIDS AND FURTHER SEPARATION OF GANGLIOSIDES BY OPTLC.

J. Pick, J. Vajda, N. Anh-Tuan, L. Leisztner, S. Hollán

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The use of the OPTLC method has been extended to the separation of the acidic fraction of the total lipid extract derived from a given blood element. This newly developed method is suitable for the interclass separation of sulphatides and gangliosides and further intraclass separation of gangliosides on the same TLC plate with step gradient development. The elutions can be performed on 10 multiplied by 10 cm (or larger) HPTLC plates with 13 parallels on each one. The chromatograms were stained either with orcinol-H//2SO//4 to show class separation (in this case only a single isocratic elution was performed) or with resorcinol-HCl reagent to visualize the ganglioside intraclass separation. The chromatograms were evaluated by spectrodensitometric scanning and the reproducibility of the separation was determined.

Original languageEnglish
Pages (from-to)2777-2791
Number of pages15
JournalJournal of Liquid Chromatography
Volume7
Issue number14
Publication statusPublished - Dec 1984

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Gangliosides
Glycolipids
Fractionation
Sulfoglycosphingolipids
Lipids
Blood
Scanning

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

CLASS FRACTIONATION OF ACIDIC GLYCOLIPIDS AND FURTHER SEPARATION OF GANGLIOSIDES BY OPTLC. / Pick, J.; Vajda, J.; Anh-Tuan, N.; Leisztner, L.; Hollán, S.

In: Journal of Liquid Chromatography, Vol. 7, No. 14, 12.1984, p. 2777-2791.

Research output: Contribution to journalArticle

Pick, J. ; Vajda, J. ; Anh-Tuan, N. ; Leisztner, L. ; Hollán, S. / CLASS FRACTIONATION OF ACIDIC GLYCOLIPIDS AND FURTHER SEPARATION OF GANGLIOSIDES BY OPTLC. In: Journal of Liquid Chromatography. 1984 ; Vol. 7, No. 14. pp. 2777-2791.
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