Citrinin-induced fluidization of the plasma membrane of the fission yeast Schizosaccharomyces pombe

Ágnes Blaskó, Nóra Mike, P. Gróf, Zoltán Gazdag, Zsuzsanna Czibulya, L. Nagy, S. Kunsági-Máté, M. Pesti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Citrinin (CTN) is a toxic fungal metabolite that is a hazardous contaminant of foods and feeds. In the present study, its acute toxicity and effects on the plasma membrane of Schizosaccharomyces pombe were investigated. The minimum inhibitory concentration of CTN against the yeast cells proved to be 500μM. Treatment with 0, 250, 500 or 1000μM CTN for 60min resulted in a 0%, 2%, 21% or 100% decrease, respectively, in the survival rate of the cell population. Treatment of cells with 0, 100, 500 or 1000μM CTN for 20min induced decrease in the phase-transition temperature of the 5-doxylstearic acid-labeled plasma membrane to 16.51, 16.04, 14.18 or 13.98°C, respectively as measured by electron paramagnetic resonance spectroscopy. This perturbation was accompanied by the efflux of essential K+ from the cells. The existence of an interaction between CTN and glutathione was detected for the first time by spectrofluorometry. Our observations may suggest a direct interaction of CTN with the free sulfhydryl groups of the integral proteins of the plasma membrane, leading to dose-dependent membrane fluidization. The change in fluidity disturbed the ionic homeostasis, contributing to the death of the cells, which is a novel aspect of CTN cytotoxicity.

Original languageEnglish
Pages (from-to)636-642
Number of pages7
JournalFood and Chemical Toxicology
Volume59
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Citrinin
citrinin
Schizosaccharomyces
Schizosaccharomyces pombe
Fluidization
Cell membranes
Yeast
plasma membrane
Cell Membrane
Cells
fluorescence emission spectroscopy
cells
sulfhydryl groups
feed contamination
Fluidity
Transition Temperature
Poisons
Fluorescence Spectrometry
Phase Transition
electron paramagnetic resonance spectroscopy

Keywords

  • Citrinin
  • EPR
  • Glutathione
  • Plasma membrane
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Citrinin-induced fluidization of the plasma membrane of the fission yeast Schizosaccharomyces pombe. / Blaskó, Ágnes; Mike, Nóra; Gróf, P.; Gazdag, Zoltán; Czibulya, Zsuzsanna; Nagy, L.; Kunsági-Máté, S.; Pesti, M.

In: Food and Chemical Toxicology, Vol. 59, 09.2013, p. 636-642.

Research output: Contribution to journalArticle

@article{0d91a8301ca34d998a558368ed332faa,
title = "Citrinin-induced fluidization of the plasma membrane of the fission yeast Schizosaccharomyces pombe",
abstract = "Citrinin (CTN) is a toxic fungal metabolite that is a hazardous contaminant of foods and feeds. In the present study, its acute toxicity and effects on the plasma membrane of Schizosaccharomyces pombe were investigated. The minimum inhibitory concentration of CTN against the yeast cells proved to be 500μM. Treatment with 0, 250, 500 or 1000μM CTN for 60min resulted in a 0{\%}, 2{\%}, 21{\%} or 100{\%} decrease, respectively, in the survival rate of the cell population. Treatment of cells with 0, 100, 500 or 1000μM CTN for 20min induced decrease in the phase-transition temperature of the 5-doxylstearic acid-labeled plasma membrane to 16.51, 16.04, 14.18 or 13.98°C, respectively as measured by electron paramagnetic resonance spectroscopy. This perturbation was accompanied by the efflux of essential K+ from the cells. The existence of an interaction between CTN and glutathione was detected for the first time by spectrofluorometry. Our observations may suggest a direct interaction of CTN with the free sulfhydryl groups of the integral proteins of the plasma membrane, leading to dose-dependent membrane fluidization. The change in fluidity disturbed the ionic homeostasis, contributing to the death of the cells, which is a novel aspect of CTN cytotoxicity.",
keywords = "Citrinin, EPR, Glutathione, Plasma membrane, Schizosaccharomyces pombe",
author = "{\'A}gnes Blask{\'o} and N{\'o}ra Mike and P. Gr{\'o}f and Zolt{\'a}n Gazdag and Zsuzsanna Czibulya and L. Nagy and S. Kuns{\'a}gi-M{\'a}t{\'e} and M. Pesti",
year = "2013",
month = "9",
doi = "10.1016/j.fct.2013.07.006",
language = "English",
volume = "59",
pages = "636--642",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Citrinin-induced fluidization of the plasma membrane of the fission yeast Schizosaccharomyces pombe

AU - Blaskó, Ágnes

AU - Mike, Nóra

AU - Gróf, P.

AU - Gazdag, Zoltán

AU - Czibulya, Zsuzsanna

AU - Nagy, L.

AU - Kunsági-Máté, S.

AU - Pesti, M.

PY - 2013/9

Y1 - 2013/9

N2 - Citrinin (CTN) is a toxic fungal metabolite that is a hazardous contaminant of foods and feeds. In the present study, its acute toxicity and effects on the plasma membrane of Schizosaccharomyces pombe were investigated. The minimum inhibitory concentration of CTN against the yeast cells proved to be 500μM. Treatment with 0, 250, 500 or 1000μM CTN for 60min resulted in a 0%, 2%, 21% or 100% decrease, respectively, in the survival rate of the cell population. Treatment of cells with 0, 100, 500 or 1000μM CTN for 20min induced decrease in the phase-transition temperature of the 5-doxylstearic acid-labeled plasma membrane to 16.51, 16.04, 14.18 or 13.98°C, respectively as measured by electron paramagnetic resonance spectroscopy. This perturbation was accompanied by the efflux of essential K+ from the cells. The existence of an interaction between CTN and glutathione was detected for the first time by spectrofluorometry. Our observations may suggest a direct interaction of CTN with the free sulfhydryl groups of the integral proteins of the plasma membrane, leading to dose-dependent membrane fluidization. The change in fluidity disturbed the ionic homeostasis, contributing to the death of the cells, which is a novel aspect of CTN cytotoxicity.

AB - Citrinin (CTN) is a toxic fungal metabolite that is a hazardous contaminant of foods and feeds. In the present study, its acute toxicity and effects on the plasma membrane of Schizosaccharomyces pombe were investigated. The minimum inhibitory concentration of CTN against the yeast cells proved to be 500μM. Treatment with 0, 250, 500 or 1000μM CTN for 60min resulted in a 0%, 2%, 21% or 100% decrease, respectively, in the survival rate of the cell population. Treatment of cells with 0, 100, 500 or 1000μM CTN for 20min induced decrease in the phase-transition temperature of the 5-doxylstearic acid-labeled plasma membrane to 16.51, 16.04, 14.18 or 13.98°C, respectively as measured by electron paramagnetic resonance spectroscopy. This perturbation was accompanied by the efflux of essential K+ from the cells. The existence of an interaction between CTN and glutathione was detected for the first time by spectrofluorometry. Our observations may suggest a direct interaction of CTN with the free sulfhydryl groups of the integral proteins of the plasma membrane, leading to dose-dependent membrane fluidization. The change in fluidity disturbed the ionic homeostasis, contributing to the death of the cells, which is a novel aspect of CTN cytotoxicity.

KW - Citrinin

KW - EPR

KW - Glutathione

KW - Plasma membrane

KW - Schizosaccharomyces pombe

UR - http://www.scopus.com/inward/record.url?scp=84881227675&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881227675&partnerID=8YFLogxK

U2 - 10.1016/j.fct.2013.07.006

DO - 10.1016/j.fct.2013.07.006

M3 - Article

C2 - 23851147

AN - SCOPUS:84881227675

VL - 59

SP - 636

EP - 642

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

ER -