Citrinin-induced fluidization of the plasma membrane of the fission yeast Schizosaccharomyces pombe

Ágnes Blaskó, Nóra Mike, Pál Gróf, Zoltán Gazdag, Zsuzsanna Czibulya, Lívia Nagy, Sándor Kunsági-Máté, Miklós Pesti

Research output: Contribution to journalArticle

7 Citations (Scopus)


Citrinin (CTN) is a toxic fungal metabolite that is a hazardous contaminant of foods and feeds. In the present study, its acute toxicity and effects on the plasma membrane of Schizosaccharomyces pombe were investigated. The minimum inhibitory concentration of CTN against the yeast cells proved to be 500μM. Treatment with 0, 250, 500 or 1000μM CTN for 60min resulted in a 0%, 2%, 21% or 100% decrease, respectively, in the survival rate of the cell population. Treatment of cells with 0, 100, 500 or 1000μM CTN for 20min induced decrease in the phase-transition temperature of the 5-doxylstearic acid-labeled plasma membrane to 16.51, 16.04, 14.18 or 13.98°C, respectively as measured by electron paramagnetic resonance spectroscopy. This perturbation was accompanied by the efflux of essential K+ from the cells. The existence of an interaction between CTN and glutathione was detected for the first time by spectrofluorometry. Our observations may suggest a direct interaction of CTN with the free sulfhydryl groups of the integral proteins of the plasma membrane, leading to dose-dependent membrane fluidization. The change in fluidity disturbed the ionic homeostasis, contributing to the death of the cells, which is a novel aspect of CTN cytotoxicity.

Original languageEnglish
Pages (from-to)636-642
Number of pages7
JournalFood and Chemical Toxicology
Publication statusPublished - Sep 1 2013


  • Citrinin
  • EPR
  • Glutathione
  • Plasma membrane
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Fingerprint Dive into the research topics of 'Citrinin-induced fluidization of the plasma membrane of the fission yeast Schizosaccharomyces pombe'. Together they form a unique fingerprint.

  • Cite this