Citrate synthase 1 interacts with the citrate transporter of yeast mitochondria.

E. V. Grigorenko, W. C. Small, L. O. Persson, P. Srere

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have previously shown that citrate synthase binds to an intrinsic protein of the mitochondrial inner membrane (D'Souza and Srere, 1983). In this paper we present evidence that this citrate synthase binding protein is the citrate transporter. We have used citrate synthase 1 mutants of Saccharomyces cerevisiae and transformants containing citrate synthase inactivated by site-directed mutagenesis to study the effect of the CS1 protein upon mitochondrial function (Kispal and Srere). In the present study citrate uptake and oxidation were measured during state 3 conditions (presence of 200 microM ADP) in the mitochondria of several strains of Saccharomyces cerevesiae: a parental strain containing wild-type mitochondrial citrate synthase (CS1) and strains derived from a CS1 deficient strain in which the CS1 gene was disrupted by insertion of the LEU2 gene. These strains were generated from the CS1- cells by transformation with vectors encoding site-specific mutants of CS1 possessing very low levels of enzymatic activity. One such strain in this study was subsequently found to have undergone reversion to produce a strain which had activity very similar to wild type. Positive correlation between citrate uptake and the rate of citrate oxidation was found, suggesting coupling of the two processes. Both mitochondrial citrate uptake and oxidation were decreased in the mutant lacking any form of CS1 protein. Reintroduction of mutagenized CS1 into yeast causes an enhancement in the rate of state 3 oxygen consumption and of citrate uptake.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)215-219
Number of pages5
JournalJournal of Molecular Recognition
Volume3
Issue number5-6
Publication statusPublished - Oct 1990

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Citrate (si)-Synthase
Mitochondria
Citric Acid
Yeast
Yeasts
Proteins
Oxidation
Genes
Saccharomyces
Insertional Mutagenesis
Mitochondrial Proteins
Administrative data processing
Mitochondrial Membranes
Mutagenesis
Site-Directed Mutagenesis
Oxygen Consumption
Adenosine Diphosphate
Saccharomyces cerevisiae
Carrier Proteins
citrate-binding transport protein

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology
  • Computer Vision and Pattern Recognition
  • Immunology

Cite this

Citrate synthase 1 interacts with the citrate transporter of yeast mitochondria. / Grigorenko, E. V.; Small, W. C.; Persson, L. O.; Srere, P.

In: Journal of Molecular Recognition, Vol. 3, No. 5-6, 10.1990, p. 215-219.

Research output: Contribution to journalArticle

Grigorenko, E. V. ; Small, W. C. ; Persson, L. O. ; Srere, P. / Citrate synthase 1 interacts with the citrate transporter of yeast mitochondria. In: Journal of Molecular Recognition. 1990 ; Vol. 3, No. 5-6. pp. 215-219.
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