Circularly permuted variants of two CG-specific prokaryotic DNA methyltransferases

Pál Albert, Bence Varga, Nikolett Zsibrita, A. Kiss

Research output: Contribution to journalArticle

Abstract

The highly similar prokaryotic DNA (cytosine-5) methyltransferases (C5-MTases) M.MpeI and M.SssI share the specificity of eukaryotic C5-MTases (5’-CG), and can be useful research tools in the study of eukaryotic DNA methylation and epigenetic regulation. In an effort to improve the stability and solubility of complementing fragments of the two MTases, genes encoding circularly permuted (CP) variants of M.MpeI and M.SssI were created, and cloned in a plasmid vector downstream of an arabinose-inducible promoter. MTase activity of the CP variants was tested by digestion of the plasmids with methylation-sensitive restriction enzymes. Eleven of the fourteen M.MpeI permutants and six of the seven M.SssI permutants had detectable MTase activity as indicated by the full or partial protection of the plasmid carrying the cpMTase gene. Permutants cp62M.MpeI and cp58M.SssI, in which the new N-termini are located between conserved motifs II and III, had by far the highest activity. The activity of cp62M.MpeI was comparable to the activity of wild-type M.MpeI. Based on the location of the split sites, the permutants possessing MTase activity can be classified in ten types. Although most permutation sites were designed to fall outside of conserved motifs, and the MTase activity of the permutants measured in cell extracts was in most cases substantially lower than that of the wild-type enzyme, the high proportion of circular permutation topologies compatible with MTase activity is remarkable, and is a new evidence for the structural plasticity of C5-MTases. A computer search of the REBASE database identified putative C5-MTases with CP arrangement. Interestingly, all natural circularly permuted C5-MTases appear to represent only one of the ten types of permutation topology created in this work.

Original languageEnglish
Article numbere0197232
JournalPLoS One
Volume13
Issue number5
DOIs
Publication statusPublished - May 1 2018

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cytosine
methyltransferases
Methyltransferases
Plasmids
DNA
DNA (Cytosine-5-)-Methyltransferase
topology
Topology
plasmids
Arabinose
Gene encoding
Methylation
DNA Methylation
Enzymes
plasmid vectors
Cell Extracts
Epigenomics
Solubility
Genes
Plasticity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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Circularly permuted variants of two CG-specific prokaryotic DNA methyltransferases. / Albert, Pál; Varga, Bence; Zsibrita, Nikolett; Kiss, A.

In: PLoS One, Vol. 13, No. 5, e0197232, 01.05.2018.

Research output: Contribution to journalArticle

Albert, Pál ; Varga, Bence ; Zsibrita, Nikolett ; Kiss, A. / Circularly permuted variants of two CG-specific prokaryotic DNA methyltransferases. In: PLoS One. 2018 ; Vol. 13, No. 5.
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