Circadian and light regulated expression of CBFs and their upstream signalling genes in barley

Krisztián Gierczik, Aliz Novák, Mohamed Ahres, András Székely, Alexandra Soltész, Ákos Boldizsár, Zsolt Gulyás, Balázs Kalapos, István Monostori, László Kozma-Bognár, Gábor Galiba, Attila Vágújfalvi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

CBF (C-repeat binding factor) transcription factors show high expression levels in response to cold; moreover, they play a key regulatory role in cold acclimation processes. Recently, however, more and more information has led to the conclusion that, apart from cold, light— including its spectra—also has a crucial role in regulating CBF expression. Earlier, studies established that the expression patterns of some of these regulatory genes follow circadian rhythms. To understand more of this complex acclimation process, we studied the expression patterns of the signal transducing pathways, including signal perception, the circadian clock and phospholipid signalling pathways, upstream of the CBF gene regulatory hub. To exclude the confounding effect of cold, experiments were carried out at 22 °C. Our results show that the expression of genes implicated in the phospholipid signalling pathway follow a circadian rhythm. We demonstrated that, from among the tested CBF genes expressed in Hordeum vulgare (Hv) under our conditions, only the members of the HvCBF4-phylogenetic subgroup showed a circadian pattern. We found that the HvCBF4-subgroup genes were expressed late in the afternoon or early in the night. We also determined the expression changes under supplemental far-red illumination and established that the transcript accumulation had appeared four hours earlier and more intensely in several cases. Based on our results, we propose a model to illustrate the effect of the circadian clock and the quality of the light on the elements of signalling pathways upstream of the HvCBFs, thus integrating the complex regulation of the early cellular responses, which finally lead to an elevated abiotic stress tolerance.

Original languageEnglish
Article number1828
JournalInternational journal of molecular sciences
Volume18
Issue number8
DOIs
Publication statusPublished - Aug 22 2017

Keywords

  • Barley
  • Calcium signalling
  • Circadian rhythm
  • Gene expression
  • HvCBF-phylogenetic subgroups
  • Light quality
  • Low red/far-red ratio

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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    Gierczik, K., Novák, A., Ahres, M., Székely, A., Soltész, A., Boldizsár, Á., Gulyás, Z., Kalapos, B., Monostori, I., Kozma-Bognár, L., Galiba, G., & Vágújfalvi, A. (2017). Circadian and light regulated expression of CBFs and their upstream signalling genes in barley. International journal of molecular sciences, 18(8), [1828]. https://doi.org/10.3390/ijms18081828