Ciprofloxacin encapsulation into giant unilamellar vesicles: Membrane binding and release

Nóra Kaszás, Tamás Bozó, Marianna Budai, Pál Gróf

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study aimed at investigating some respects of binding and interaction between water-soluble drugs and liposomal carrier systems depending on their size and lamellarity. As model substance, ciprofloxacin hydrochloride (CPFX) was incorporated into giant unilamellar vesicles (GUVs) to study their CPFX encapsulation/binding capacity. To characterize molecular interactions of various CPFX microspecies with lipid bilayer, zeta potential and electron paramagnetic resonance (EPR) spectroscopy measurements were performed. The increase of the zeta potential at pH 5.4 but no change at pH 7.2 was interpreted in terms of the CPFX microspecies' distribution at the two pH values. EPR observations showed an increased fluidity because of CPFX binding to GUVs. We worked out and applied a three-compartment dialysis model to separately determine the rate of drug diffusion through the liposomal membrane. Equilibrium dialysis showed (a) different permeation of CPFX through the membranes of GUVs and multilamellar vesicles (MLVs), with characteristic half-lives of 54.4 and 18.1 h, respectively; and (b) increased retention of CPFX in case of GUVs with released amounts of 70% compared with about 97% in case of MLVs. Our results may provide further details for efficient design of liposomal formulations incorporating water-soluble drugs.

Original languageEnglish
Pages (from-to)694-705
Number of pages12
JournalJournal of pharmaceutical sciences
Volume102
Issue number2
DOIs
Publication statusPublished - Feb 2013

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Keywords

  • Encapsulation
  • Formulation
  • Formulation vehicle
  • Giant vesicles
  • Liposomal permeation
  • Liposomes
  • Permeability
  • Three-compartment diffusion model
  • Zeta potential

ASJC Scopus subject areas

  • Pharmaceutical Science

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