Chronic ethanol exposure impairs neuronal nitric oxide synthase in the rat intestine

Monika Krecsmarik, F. Izbéki, Mária Bagyánszki, Nikolett Linke, Nikolett Bódi, J. Kaszaki, Zója Katarova, Áron Szabó, Éva Fekete, T. Wittmann

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Nitric oxide (NO), synthesized by neuronal (nNOS), endothelial (eNOS), and inducible (iNOS) nitric oxide synthases, plays an essential role in the physiological functions of the gastrointestinal (GI) tract. Chronic ethanol intake has been shown to interfere with several of these physiological functions, leading to the pathological alterations observed in alcoholic individuals. Our aim therefore was to investigate the effects of chronic ethanol consumption on NOS isoforms in different GI segments. Methods: Rats received either 20% aqueous ethanol solution or water for 8 weeks. Tissue samples of the duodenum, jejunum, ileum, and colon of the rats were used for measurement of the NOS activity, protein content, and nNOS immunohistochemistry. Anti-HuC/D immunohistochemistry was used to determine the total number of neurons. Results: Measurement of the physiological constitutive NOS (cNOS) activity revealed a 20 times higher activity in the colon than in the small intestine and after chronic ethanol treatment demonstrated a significant decrease in the jejunum, ileum, and colon, while in the duodenum it remained unchanged compared with the control group. The physiological iNOS activity was higher in the ileum and colon than in the duodenum and jejunum, and these levels were not significantly affected by ethanol. Neuronal nitric oxide synthase immunohistochemistry revealed a significant decrease in the numbers of immunostained cells in all investigated intestinal segments, while the total number of myenteric neurons remained constant. The nNOS protein content measured by Western blotting indicated a significant decrease in the colon after ethanol consumption, while in other intestinal segments change was not detectable. Conclusions: This study has demonstrated for the first time that chronic ethanol consumption has a differential effect on NOS activity, NOS protein content, and the number of nitrergic neurons in different intestinal segments, suggesting that chronic ethanol administration affects the NO pathways in the enteric nervous system.

Original languageEnglish
Pages (from-to)967-973
Number of pages7
JournalAlcoholism: Clinical and Experimental Research
Volume30
Issue number6
DOIs
Publication statusPublished - Jun 2006

Fingerprint

Nitric Oxide Synthase Type I
Intestines
Rats
Ethanol
Colon
Jejunum
Ileum
Duodenum
Neurons
Immunohistochemistry
Nitric Oxide
Nitrergic Neurons
Enteric Nervous System
Proteins
Neurology
Nitric Oxide Synthase Type II
Nitric Oxide Synthase
Small Intestine
Gastrointestinal Tract
Protein Isoforms

Keywords

  • Chronic Ethanol Consumption
  • Immunohistochemistry
  • Intestine
  • Nitric Oxide Synthase
  • NOS Activity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Chronic ethanol exposure impairs neuronal nitric oxide synthase in the rat intestine. / Krecsmarik, Monika; Izbéki, F.; Bagyánszki, Mária; Linke, Nikolett; Bódi, Nikolett; Kaszaki, J.; Katarova, Zója; Szabó, Áron; Fekete, Éva; Wittmann, T.

In: Alcoholism: Clinical and Experimental Research, Vol. 30, No. 6, 06.2006, p. 967-973.

Research output: Contribution to journalArticle

Krecsmarik, Monika ; Izbéki, F. ; Bagyánszki, Mária ; Linke, Nikolett ; Bódi, Nikolett ; Kaszaki, J. ; Katarova, Zója ; Szabó, Áron ; Fekete, Éva ; Wittmann, T. / Chronic ethanol exposure impairs neuronal nitric oxide synthase in the rat intestine. In: Alcoholism: Clinical and Experimental Research. 2006 ; Vol. 30, No. 6. pp. 967-973.
@article{c750b554562441659a81370a74270022,
title = "Chronic ethanol exposure impairs neuronal nitric oxide synthase in the rat intestine",
abstract = "Background: Nitric oxide (NO), synthesized by neuronal (nNOS), endothelial (eNOS), and inducible (iNOS) nitric oxide synthases, plays an essential role in the physiological functions of the gastrointestinal (GI) tract. Chronic ethanol intake has been shown to interfere with several of these physiological functions, leading to the pathological alterations observed in alcoholic individuals. Our aim therefore was to investigate the effects of chronic ethanol consumption on NOS isoforms in different GI segments. Methods: Rats received either 20{\%} aqueous ethanol solution or water for 8 weeks. Tissue samples of the duodenum, jejunum, ileum, and colon of the rats were used for measurement of the NOS activity, protein content, and nNOS immunohistochemistry. Anti-HuC/D immunohistochemistry was used to determine the total number of neurons. Results: Measurement of the physiological constitutive NOS (cNOS) activity revealed a 20 times higher activity in the colon than in the small intestine and after chronic ethanol treatment demonstrated a significant decrease in the jejunum, ileum, and colon, while in the duodenum it remained unchanged compared with the control group. The physiological iNOS activity was higher in the ileum and colon than in the duodenum and jejunum, and these levels were not significantly affected by ethanol. Neuronal nitric oxide synthase immunohistochemistry revealed a significant decrease in the numbers of immunostained cells in all investigated intestinal segments, while the total number of myenteric neurons remained constant. The nNOS protein content measured by Western blotting indicated a significant decrease in the colon after ethanol consumption, while in other intestinal segments change was not detectable. Conclusions: This study has demonstrated for the first time that chronic ethanol consumption has a differential effect on NOS activity, NOS protein content, and the number of nitrergic neurons in different intestinal segments, suggesting that chronic ethanol administration affects the NO pathways in the enteric nervous system.",
keywords = "Chronic Ethanol Consumption, Immunohistochemistry, Intestine, Nitric Oxide Synthase, NOS Activity",
author = "Monika Krecsmarik and F. Izb{\'e}ki and M{\'a}ria Bagy{\'a}nszki and Nikolett Linke and Nikolett B{\'o}di and J. Kaszaki and Z{\'o}ja Katarova and {\'A}ron Szab{\'o} and {\'E}va Fekete and T. Wittmann",
year = "2006",
month = "6",
doi = "10.1111/j.1530-0277.2006.00110.x",
language = "English",
volume = "30",
pages = "967--973",
journal = "Alcoholism: Clinical and Experimental Research",
issn = "0145-6008",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Chronic ethanol exposure impairs neuronal nitric oxide synthase in the rat intestine

AU - Krecsmarik, Monika

AU - Izbéki, F.

AU - Bagyánszki, Mária

AU - Linke, Nikolett

AU - Bódi, Nikolett

AU - Kaszaki, J.

AU - Katarova, Zója

AU - Szabó, Áron

AU - Fekete, Éva

AU - Wittmann, T.

PY - 2006/6

Y1 - 2006/6

N2 - Background: Nitric oxide (NO), synthesized by neuronal (nNOS), endothelial (eNOS), and inducible (iNOS) nitric oxide synthases, plays an essential role in the physiological functions of the gastrointestinal (GI) tract. Chronic ethanol intake has been shown to interfere with several of these physiological functions, leading to the pathological alterations observed in alcoholic individuals. Our aim therefore was to investigate the effects of chronic ethanol consumption on NOS isoforms in different GI segments. Methods: Rats received either 20% aqueous ethanol solution or water for 8 weeks. Tissue samples of the duodenum, jejunum, ileum, and colon of the rats were used for measurement of the NOS activity, protein content, and nNOS immunohistochemistry. Anti-HuC/D immunohistochemistry was used to determine the total number of neurons. Results: Measurement of the physiological constitutive NOS (cNOS) activity revealed a 20 times higher activity in the colon than in the small intestine and after chronic ethanol treatment demonstrated a significant decrease in the jejunum, ileum, and colon, while in the duodenum it remained unchanged compared with the control group. The physiological iNOS activity was higher in the ileum and colon than in the duodenum and jejunum, and these levels were not significantly affected by ethanol. Neuronal nitric oxide synthase immunohistochemistry revealed a significant decrease in the numbers of immunostained cells in all investigated intestinal segments, while the total number of myenteric neurons remained constant. The nNOS protein content measured by Western blotting indicated a significant decrease in the colon after ethanol consumption, while in other intestinal segments change was not detectable. Conclusions: This study has demonstrated for the first time that chronic ethanol consumption has a differential effect on NOS activity, NOS protein content, and the number of nitrergic neurons in different intestinal segments, suggesting that chronic ethanol administration affects the NO pathways in the enteric nervous system.

AB - Background: Nitric oxide (NO), synthesized by neuronal (nNOS), endothelial (eNOS), and inducible (iNOS) nitric oxide synthases, plays an essential role in the physiological functions of the gastrointestinal (GI) tract. Chronic ethanol intake has been shown to interfere with several of these physiological functions, leading to the pathological alterations observed in alcoholic individuals. Our aim therefore was to investigate the effects of chronic ethanol consumption on NOS isoforms in different GI segments. Methods: Rats received either 20% aqueous ethanol solution or water for 8 weeks. Tissue samples of the duodenum, jejunum, ileum, and colon of the rats were used for measurement of the NOS activity, protein content, and nNOS immunohistochemistry. Anti-HuC/D immunohistochemistry was used to determine the total number of neurons. Results: Measurement of the physiological constitutive NOS (cNOS) activity revealed a 20 times higher activity in the colon than in the small intestine and after chronic ethanol treatment demonstrated a significant decrease in the jejunum, ileum, and colon, while in the duodenum it remained unchanged compared with the control group. The physiological iNOS activity was higher in the ileum and colon than in the duodenum and jejunum, and these levels were not significantly affected by ethanol. Neuronal nitric oxide synthase immunohistochemistry revealed a significant decrease in the numbers of immunostained cells in all investigated intestinal segments, while the total number of myenteric neurons remained constant. The nNOS protein content measured by Western blotting indicated a significant decrease in the colon after ethanol consumption, while in other intestinal segments change was not detectable. Conclusions: This study has demonstrated for the first time that chronic ethanol consumption has a differential effect on NOS activity, NOS protein content, and the number of nitrergic neurons in different intestinal segments, suggesting that chronic ethanol administration affects the NO pathways in the enteric nervous system.

KW - Chronic Ethanol Consumption

KW - Immunohistochemistry

KW - Intestine

KW - Nitric Oxide Synthase

KW - NOS Activity

UR - http://www.scopus.com/inward/record.url?scp=33744733421&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744733421&partnerID=8YFLogxK

U2 - 10.1111/j.1530-0277.2006.00110.x

DO - 10.1111/j.1530-0277.2006.00110.x

M3 - Article

C2 - 16737454

AN - SCOPUS:33744733421

VL - 30

SP - 967

EP - 973

JO - Alcoholism: Clinical and Experimental Research

JF - Alcoholism: Clinical and Experimental Research

SN - 0145-6008

IS - 6

ER -