Chronic 17β-estradiol pretreatment has pronociceptive effect on behavioral and morphological changes induced by orofacial formalin in ovariectomized rats

Annamária Fejes-Szabó, Eleonóra Spekker, Lilla Tar, Gábor Nagy-Grócz, Zsuzsanna Bohár, Klaudia Flóra Laborc, L. Vécsei, A. Párdutz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: The prevalence of craniofacial pain disorders show sexual dimorphism with generally more common appearance in women suggesting the influence of estradiol, but the exact cause remains unknown. The common point in the pathogenesis of these disorders is the activation of trigeminal system. One of the animal experimental models of trigeminal activation is the orofacial formalin test, in which we investigated the effect of chronic 17β-estradiol pretreatment on the trigeminal pain-related behavior and activation of trigeminal second-order neurons at the level of spinal trigeminal nucleus pars caudalis (TNC). Methods: Female Sprague Dawley rats were ovariectomized and silicone capsules were implanted subcutaneously containing cholesterol in the OVX group and 17β-estradiol and cholesterol in 1:1 ratio in the OVX+E group. We determined 17β-estradiol levels in serum 2 after the implantation of capsules. Three weeks after operation, 50 µL of physiological saline or 1.5% of formalin solution was injected subcutaneously into the right whisker pad of rats. The time spent on rubbing directed to the injected area and c-Fos immunoreactivity in TNC was measured as the formalin-induced pain-related behavior, and as the marker of pain-related neuronal activation, respectively. Results: The chronic 17β-estradiol pretreatment mimics the plasma levels of estrogen occurring in the proestrus phase and significantly increased the formalin-induced pain-related behavior and neuronal activation in TNC. Conclusion: Our results demonstrate that the chronic 17β-estradiol treatment has strong pronociceptive effect on orofacial formalin-induced inflammatory pain suggesting modulatory action of estradiol on head pain through estrogen receptors, which are present in the trigeminal system.

Original languageEnglish
Pages (from-to)2011-2021
Number of pages11
JournalJournal of Pain Research
Volume11
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Formaldehyde
Estradiol
Pain
Trigeminal Nuclei
Capsules
Cholesterol
Spinal Trigeminal Nucleus
Vibrissae
Proestrus
Facial Pain
Somatoform Disorders
Silicones
Pain Measurement
Sex Characteristics
Estrogen Receptors
Headache
Sprague Dawley Rats
Estrogens
Animal Models
Neurons

Keywords

  • C-Fos
  • Headache
  • Pain
  • Sexual dimorphism
  • Trigeminal system

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Chronic 17β-estradiol pretreatment has pronociceptive effect on behavioral and morphological changes induced by orofacial formalin in ovariectomized rats. / Fejes-Szabó, Annamária; Spekker, Eleonóra; Tar, Lilla; Nagy-Grócz, Gábor; Bohár, Zsuzsanna; Laborc, Klaudia Flóra; Vécsei, L.; Párdutz, A.

In: Journal of Pain Research, Vol. 11, 01.01.2018, p. 2011-2021.

Research output: Contribution to journalArticle

Fejes-Szabó, Annamária ; Spekker, Eleonóra ; Tar, Lilla ; Nagy-Grócz, Gábor ; Bohár, Zsuzsanna ; Laborc, Klaudia Flóra ; Vécsei, L. ; Párdutz, A. / Chronic 17β-estradiol pretreatment has pronociceptive effect on behavioral and morphological changes induced by orofacial formalin in ovariectomized rats. In: Journal of Pain Research. 2018 ; Vol. 11. pp. 2011-2021.
@article{909da10b8fab40c59dd5b7f0a0253e56,
title = "Chronic 17β-estradiol pretreatment has pronociceptive effect on behavioral and morphological changes induced by orofacial formalin in ovariectomized rats",
abstract = "Background: The prevalence of craniofacial pain disorders show sexual dimorphism with generally more common appearance in women suggesting the influence of estradiol, but the exact cause remains unknown. The common point in the pathogenesis of these disorders is the activation of trigeminal system. One of the animal experimental models of trigeminal activation is the orofacial formalin test, in which we investigated the effect of chronic 17β-estradiol pretreatment on the trigeminal pain-related behavior and activation of trigeminal second-order neurons at the level of spinal trigeminal nucleus pars caudalis (TNC). Methods: Female Sprague Dawley rats were ovariectomized and silicone capsules were implanted subcutaneously containing cholesterol in the OVX group and 17β-estradiol and cholesterol in 1:1 ratio in the OVX+E group. We determined 17β-estradiol levels in serum 2 after the implantation of capsules. Three weeks after operation, 50 µL of physiological saline or 1.5{\%} of formalin solution was injected subcutaneously into the right whisker pad of rats. The time spent on rubbing directed to the injected area and c-Fos immunoreactivity in TNC was measured as the formalin-induced pain-related behavior, and as the marker of pain-related neuronal activation, respectively. Results: The chronic 17β-estradiol pretreatment mimics the plasma levels of estrogen occurring in the proestrus phase and significantly increased the formalin-induced pain-related behavior and neuronal activation in TNC. Conclusion: Our results demonstrate that the chronic 17β-estradiol treatment has strong pronociceptive effect on orofacial formalin-induced inflammatory pain suggesting modulatory action of estradiol on head pain through estrogen receptors, which are present in the trigeminal system.",
keywords = "C-Fos, Headache, Pain, Sexual dimorphism, Trigeminal system",
author = "Annam{\'a}ria Fejes-Szab{\'o} and Eleon{\'o}ra Spekker and Lilla Tar and G{\'a}bor Nagy-Gr{\'o}cz and Zsuzsanna Boh{\'a}r and Laborc, {Klaudia Fl{\'o}ra} and L. V{\'e}csei and A. P{\'a}rdutz",
year = "2018",
month = "1",
day = "1",
doi = "10.2147/JPR.S165969",
language = "English",
volume = "11",
pages = "2011--2021",
journal = "Journal of Pain Research",
issn = "1178-7090",
publisher = "Dove Medical Press Ltd.",

}

TY - JOUR

T1 - Chronic 17β-estradiol pretreatment has pronociceptive effect on behavioral and morphological changes induced by orofacial formalin in ovariectomized rats

AU - Fejes-Szabó, Annamária

AU - Spekker, Eleonóra

AU - Tar, Lilla

AU - Nagy-Grócz, Gábor

AU - Bohár, Zsuzsanna

AU - Laborc, Klaudia Flóra

AU - Vécsei, L.

AU - Párdutz, A.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: The prevalence of craniofacial pain disorders show sexual dimorphism with generally more common appearance in women suggesting the influence of estradiol, but the exact cause remains unknown. The common point in the pathogenesis of these disorders is the activation of trigeminal system. One of the animal experimental models of trigeminal activation is the orofacial formalin test, in which we investigated the effect of chronic 17β-estradiol pretreatment on the trigeminal pain-related behavior and activation of trigeminal second-order neurons at the level of spinal trigeminal nucleus pars caudalis (TNC). Methods: Female Sprague Dawley rats were ovariectomized and silicone capsules were implanted subcutaneously containing cholesterol in the OVX group and 17β-estradiol and cholesterol in 1:1 ratio in the OVX+E group. We determined 17β-estradiol levels in serum 2 after the implantation of capsules. Three weeks after operation, 50 µL of physiological saline or 1.5% of formalin solution was injected subcutaneously into the right whisker pad of rats. The time spent on rubbing directed to the injected area and c-Fos immunoreactivity in TNC was measured as the formalin-induced pain-related behavior, and as the marker of pain-related neuronal activation, respectively. Results: The chronic 17β-estradiol pretreatment mimics the plasma levels of estrogen occurring in the proestrus phase and significantly increased the formalin-induced pain-related behavior and neuronal activation in TNC. Conclusion: Our results demonstrate that the chronic 17β-estradiol treatment has strong pronociceptive effect on orofacial formalin-induced inflammatory pain suggesting modulatory action of estradiol on head pain through estrogen receptors, which are present in the trigeminal system.

AB - Background: The prevalence of craniofacial pain disorders show sexual dimorphism with generally more common appearance in women suggesting the influence of estradiol, but the exact cause remains unknown. The common point in the pathogenesis of these disorders is the activation of trigeminal system. One of the animal experimental models of trigeminal activation is the orofacial formalin test, in which we investigated the effect of chronic 17β-estradiol pretreatment on the trigeminal pain-related behavior and activation of trigeminal second-order neurons at the level of spinal trigeminal nucleus pars caudalis (TNC). Methods: Female Sprague Dawley rats were ovariectomized and silicone capsules were implanted subcutaneously containing cholesterol in the OVX group and 17β-estradiol and cholesterol in 1:1 ratio in the OVX+E group. We determined 17β-estradiol levels in serum 2 after the implantation of capsules. Three weeks after operation, 50 µL of physiological saline or 1.5% of formalin solution was injected subcutaneously into the right whisker pad of rats. The time spent on rubbing directed to the injected area and c-Fos immunoreactivity in TNC was measured as the formalin-induced pain-related behavior, and as the marker of pain-related neuronal activation, respectively. Results: The chronic 17β-estradiol pretreatment mimics the plasma levels of estrogen occurring in the proestrus phase and significantly increased the formalin-induced pain-related behavior and neuronal activation in TNC. Conclusion: Our results demonstrate that the chronic 17β-estradiol treatment has strong pronociceptive effect on orofacial formalin-induced inflammatory pain suggesting modulatory action of estradiol on head pain through estrogen receptors, which are present in the trigeminal system.

KW - C-Fos

KW - Headache

KW - Pain

KW - Sexual dimorphism

KW - Trigeminal system

UR - http://www.scopus.com/inward/record.url?scp=85058805753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058805753&partnerID=8YFLogxK

U2 - 10.2147/JPR.S165969

DO - 10.2147/JPR.S165969

M3 - Article

AN - SCOPUS:85058805753

VL - 11

SP - 2011

EP - 2021

JO - Journal of Pain Research

JF - Journal of Pain Research

SN - 1178-7090

ER -