Chromosome instability of wheat/barley ditelosomic addition lines in tissue culture

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Abstract

The chromosome instability of some Chinese Spring/Betzes addition lines was studied in sexually propagated plants and in plants propagated in tissue culture. A total of 197 regenerant plants were raised after propagation in tissue culture from six Chinese Spring/Betzes wheat/barley ditelosomic addition lines (1Hβ 3HS, 3HL, 6HS, 6HL and 7HS) isolated by Islam (1983). The highest regeneration frequency was detected in the 3HL and 6HL addition lines. Cytological analyses of seed obtained on the regenerants showed that 49.8% of the seeds examined contained 42+2t chromosomes, 46.6% had 42 chromosomes, while 3.6% contained various numbers of chromosomes (42+1t, 41, 43). The loss of the additioned pair of telocentric barley chromosomes was most frequent in progeny regenerated from the 3HL line, where 88.4% of the seed examined had a chromosome number of 42. However, when propagated sexually 3HL proved to be one of the most stable wheat/barley ditelosomic addition lines; 93.1% of the seed analysed contained 42+2t chromosomes. The least stable addition line among the sexually propagated plants was the 7HS addition line, where only 15.4 % of the seed contained the additioned barley telocentric chromosomes.

Original languageEnglish
Pages (from-to)275-281
Number of pages7
JournalCereal Research Communications
Volume24
Issue number3
Publication statusPublished - 1996

Fingerprint

Chromosomal Instability
Hordeum
Triticum
tissue culture
Chromosomes
barley
chromosomes
wheat
Seeds
seeds
telocentric chromosomes
Islam
spring wheat
chromosome number
plant tissues
Regeneration

Keywords

  • barley
  • chromosome instability
  • ditelosomic addition lines
  • tissue culture
  • wheat

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Chromosome instability of wheat/barley ditelosomic addition lines in tissue culture",
abstract = "The chromosome instability of some Chinese Spring/Betzes addition lines was studied in sexually propagated plants and in plants propagated in tissue culture. A total of 197 regenerant plants were raised after propagation in tissue culture from six Chinese Spring/Betzes wheat/barley ditelosomic addition lines (1Hβ 3HS, 3HL, 6HS, 6HL and 7HS) isolated by Islam (1983). The highest regeneration frequency was detected in the 3HL and 6HL addition lines. Cytological analyses of seed obtained on the regenerants showed that 49.8{\%} of the seeds examined contained 42+2t chromosomes, 46.6{\%} had 42 chromosomes, while 3.6{\%} contained various numbers of chromosomes (42+1t, 41, 43). The loss of the additioned pair of telocentric barley chromosomes was most frequent in progeny regenerated from the 3HL line, where 88.4{\%} of the seed examined had a chromosome number of 42. However, when propagated sexually 3HL proved to be one of the most stable wheat/barley ditelosomic addition lines; 93.1{\%} of the seed analysed contained 42+2t chromosomes. The least stable addition line among the sexually propagated plants was the 7HS addition line, where only 15.4 {\%} of the seed contained the additioned barley telocentric chromosomes.",
keywords = "barley, chromosome instability, ditelosomic addition lines, tissue culture, wheat",
author = "M. Moln{\'a}r-L{\'a}ng and B. Koszegi and G. Linc and G. Galiba and J. Sutka",
year = "1996",
language = "English",
volume = "24",
pages = "275--281",
journal = "Cereal Research Communications",
issn = "0133-3720",
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TY - JOUR

T1 - Chromosome instability of wheat/barley ditelosomic addition lines in tissue culture

AU - Molnár-Láng, M.

AU - Koszegi, B.

AU - Linc, G.

AU - Galiba, G.

AU - Sutka, J.

PY - 1996

Y1 - 1996

N2 - The chromosome instability of some Chinese Spring/Betzes addition lines was studied in sexually propagated plants and in plants propagated in tissue culture. A total of 197 regenerant plants were raised after propagation in tissue culture from six Chinese Spring/Betzes wheat/barley ditelosomic addition lines (1Hβ 3HS, 3HL, 6HS, 6HL and 7HS) isolated by Islam (1983). The highest regeneration frequency was detected in the 3HL and 6HL addition lines. Cytological analyses of seed obtained on the regenerants showed that 49.8% of the seeds examined contained 42+2t chromosomes, 46.6% had 42 chromosomes, while 3.6% contained various numbers of chromosomes (42+1t, 41, 43). The loss of the additioned pair of telocentric barley chromosomes was most frequent in progeny regenerated from the 3HL line, where 88.4% of the seed examined had a chromosome number of 42. However, when propagated sexually 3HL proved to be one of the most stable wheat/barley ditelosomic addition lines; 93.1% of the seed analysed contained 42+2t chromosomes. The least stable addition line among the sexually propagated plants was the 7HS addition line, where only 15.4 % of the seed contained the additioned barley telocentric chromosomes.

AB - The chromosome instability of some Chinese Spring/Betzes addition lines was studied in sexually propagated plants and in plants propagated in tissue culture. A total of 197 regenerant plants were raised after propagation in tissue culture from six Chinese Spring/Betzes wheat/barley ditelosomic addition lines (1Hβ 3HS, 3HL, 6HS, 6HL and 7HS) isolated by Islam (1983). The highest regeneration frequency was detected in the 3HL and 6HL addition lines. Cytological analyses of seed obtained on the regenerants showed that 49.8% of the seeds examined contained 42+2t chromosomes, 46.6% had 42 chromosomes, while 3.6% contained various numbers of chromosomes (42+1t, 41, 43). The loss of the additioned pair of telocentric barley chromosomes was most frequent in progeny regenerated from the 3HL line, where 88.4% of the seed examined had a chromosome number of 42. However, when propagated sexually 3HL proved to be one of the most stable wheat/barley ditelosomic addition lines; 93.1% of the seed analysed contained 42+2t chromosomes. The least stable addition line among the sexually propagated plants was the 7HS addition line, where only 15.4 % of the seed contained the additioned barley telocentric chromosomes.

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KW - tissue culture

KW - wheat

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