Chromosome and plasmid-encoded N-acyl homoserine lactones produced by Agrobacterium vitis wildtype and mutants that differ in their interactions with grape and tobacco

Yaxin Li, Matthew R. Gronquist, Guixia Hao, Michele R. Holden, Anatol Eberhard, Russell A. Scott, Michael A. Savka, E. Szegedi, S. Süle, Thomas J. Burr

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Agrobacterium vitis causes crown gall disease on grapevines. It also induces a specific necrosis on grape roots and a hypersensitive response (HR) on tobacco that are regulated by a complex quorum-sensing regulatory system. Strain F2/5 produces at least six N-acyl-homoserine lactones (AHLs) that function as signal molecules in quorum-sensing. The AHLs differ in acyl side chain length (8-16 carbons) as determined by gas chromatography/mass spectrometry and electrospray ionization tandem mass spectrometry. Mutant derivatives of F2/5 differ in ability to cause necrosis and the HR and show variable AHL profiles as determined by a thin-layer chromatography/biosensor assay. All wildtype A. vitis strains revealed the presence of long-chain AHLs regardless of tumorigenicity or ability to cause the HR. Whereas genes encoding long-chain AHLs are predicted to reside on the F2/5 chromosome, the determinants for short-chain AHLs were shown to be located on conjugal plasmids.

Original languageEnglish
Pages (from-to)284-290
Number of pages7
JournalPhysiological and Molecular Plant Pathology
Volume67
Issue number6
DOIs
Publication statusPublished - Dec 2006

Fingerprint

Rhizobium vitis
Acyl-Butyrolactones
homoserine
Agrobacterium
Vitis
lactones
Tobacco
grapes
plasmids
Plasmids
tobacco
Chromosomes
chromosomes
mutants
hypersensitive response
Quorum Sensing
quorum sensing
necrosis
Necrosis
Plant Tumors

Keywords

  • Agrobacterium tumefaciens
  • Autoinducer
  • Hypersensitive response
  • Quorum-sensing
  • Rhizobiaceae

ASJC Scopus subject areas

  • Plant Science

Cite this

Chromosome and plasmid-encoded N-acyl homoserine lactones produced by Agrobacterium vitis wildtype and mutants that differ in their interactions with grape and tobacco. / Li, Yaxin; Gronquist, Matthew R.; Hao, Guixia; Holden, Michele R.; Eberhard, Anatol; Scott, Russell A.; Savka, Michael A.; Szegedi, E.; Süle, S.; Burr, Thomas J.

In: Physiological and Molecular Plant Pathology, Vol. 67, No. 6, 12.2006, p. 284-290.

Research output: Contribution to journalArticle

Li, Yaxin ; Gronquist, Matthew R. ; Hao, Guixia ; Holden, Michele R. ; Eberhard, Anatol ; Scott, Russell A. ; Savka, Michael A. ; Szegedi, E. ; Süle, S. ; Burr, Thomas J. / Chromosome and plasmid-encoded N-acyl homoserine lactones produced by Agrobacterium vitis wildtype and mutants that differ in their interactions with grape and tobacco. In: Physiological and Molecular Plant Pathology. 2006 ; Vol. 67, No. 6. pp. 284-290.
@article{e4e559dd5aa04918acdb0ccd3faa6539,
title = "Chromosome and plasmid-encoded N-acyl homoserine lactones produced by Agrobacterium vitis wildtype and mutants that differ in their interactions with grape and tobacco",
abstract = "Agrobacterium vitis causes crown gall disease on grapevines. It also induces a specific necrosis on grape roots and a hypersensitive response (HR) on tobacco that are regulated by a complex quorum-sensing regulatory system. Strain F2/5 produces at least six N-acyl-homoserine lactones (AHLs) that function as signal molecules in quorum-sensing. The AHLs differ in acyl side chain length (8-16 carbons) as determined by gas chromatography/mass spectrometry and electrospray ionization tandem mass spectrometry. Mutant derivatives of F2/5 differ in ability to cause necrosis and the HR and show variable AHL profiles as determined by a thin-layer chromatography/biosensor assay. All wildtype A. vitis strains revealed the presence of long-chain AHLs regardless of tumorigenicity or ability to cause the HR. Whereas genes encoding long-chain AHLs are predicted to reside on the F2/5 chromosome, the determinants for short-chain AHLs were shown to be located on conjugal plasmids.",
keywords = "Agrobacterium tumefaciens, Autoinducer, Hypersensitive response, Quorum-sensing, Rhizobiaceae",
author = "Yaxin Li and Gronquist, {Matthew R.} and Guixia Hao and Holden, {Michele R.} and Anatol Eberhard and Scott, {Russell A.} and Savka, {Michael A.} and E. Szegedi and S. S{\"u}le and Burr, {Thomas J.}",
year = "2006",
month = "12",
doi = "10.1016/j.pmpp.2006.04.002",
language = "English",
volume = "67",
pages = "284--290",
journal = "Physiological and Molecular Plant Pathology",
issn = "0885-5765",
publisher = "Academic Press Inc.",
number = "6",

}

TY - JOUR

T1 - Chromosome and plasmid-encoded N-acyl homoserine lactones produced by Agrobacterium vitis wildtype and mutants that differ in their interactions with grape and tobacco

AU - Li, Yaxin

AU - Gronquist, Matthew R.

AU - Hao, Guixia

AU - Holden, Michele R.

AU - Eberhard, Anatol

AU - Scott, Russell A.

AU - Savka, Michael A.

AU - Szegedi, E.

AU - Süle, S.

AU - Burr, Thomas J.

PY - 2006/12

Y1 - 2006/12

N2 - Agrobacterium vitis causes crown gall disease on grapevines. It also induces a specific necrosis on grape roots and a hypersensitive response (HR) on tobacco that are regulated by a complex quorum-sensing regulatory system. Strain F2/5 produces at least six N-acyl-homoserine lactones (AHLs) that function as signal molecules in quorum-sensing. The AHLs differ in acyl side chain length (8-16 carbons) as determined by gas chromatography/mass spectrometry and electrospray ionization tandem mass spectrometry. Mutant derivatives of F2/5 differ in ability to cause necrosis and the HR and show variable AHL profiles as determined by a thin-layer chromatography/biosensor assay. All wildtype A. vitis strains revealed the presence of long-chain AHLs regardless of tumorigenicity or ability to cause the HR. Whereas genes encoding long-chain AHLs are predicted to reside on the F2/5 chromosome, the determinants for short-chain AHLs were shown to be located on conjugal plasmids.

AB - Agrobacterium vitis causes crown gall disease on grapevines. It also induces a specific necrosis on grape roots and a hypersensitive response (HR) on tobacco that are regulated by a complex quorum-sensing regulatory system. Strain F2/5 produces at least six N-acyl-homoserine lactones (AHLs) that function as signal molecules in quorum-sensing. The AHLs differ in acyl side chain length (8-16 carbons) as determined by gas chromatography/mass spectrometry and electrospray ionization tandem mass spectrometry. Mutant derivatives of F2/5 differ in ability to cause necrosis and the HR and show variable AHL profiles as determined by a thin-layer chromatography/biosensor assay. All wildtype A. vitis strains revealed the presence of long-chain AHLs regardless of tumorigenicity or ability to cause the HR. Whereas genes encoding long-chain AHLs are predicted to reside on the F2/5 chromosome, the determinants for short-chain AHLs were shown to be located on conjugal plasmids.

KW - Agrobacterium tumefaciens

KW - Autoinducer

KW - Hypersensitive response

KW - Quorum-sensing

KW - Rhizobiaceae

UR - http://www.scopus.com/inward/record.url?scp=33746351076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746351076&partnerID=8YFLogxK

U2 - 10.1016/j.pmpp.2006.04.002

DO - 10.1016/j.pmpp.2006.04.002

M3 - Article

AN - SCOPUS:33746351076

VL - 67

SP - 284

EP - 290

JO - Physiological and Molecular Plant Pathology

JF - Physiological and Molecular Plant Pathology

SN - 0885-5765

IS - 6

ER -