Yeast chromosomal DNA was prepared under different conditions. Treatment of intact cells with proteinase K (1 mg/ml) resulted in appropriate electrophoretic karyotypes; when protoplasts were formed in situ, the presence of both sodium lauroylsarcosine and EDTA was essential. Further, the duration of cell wall lysis (12 h) and the concentrations of lytic enzymes (0.5% snail enzyme and 0.25% Novozym) had to be kept at a minimum.
|Number of pages||4|
|Publication status||Published - Oct 9 1996|
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology