Chromosomal DNA preparation from yeasts of biotechnological importance

Zs Palágyi, Á Nagy, T. Papp, L. Ferenczy, C. Vágvölgyi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Yeast chromosomal DNA was prepared under different conditions. Treatment of intact cells with proteinase K (1 mg/ml) resulted in appropriate electrophoretic karyotypes; when protoplasts were formed in situ, the presence of both sodium lauroylsarcosine and EDTA was essential. Further, the duration of cell wall lysis (12 h) and the concentrations of lytic enzymes (0.5% snail enzyme and 0.25% Novozym) had to be kept at a minimum.

Original languageEnglish
Pages (from-to)565-568
Number of pages4
JournalBiotechnology Techniques
Volume10
Issue number8
Publication statusPublished - 1996

Fingerprint

Yeast
DNA
Enzymes
Yeasts
Cells
Endopeptidase K
Protoplasts
Ethylenediaminetetraacetic acid
Snails
Karyotype
Edetic Acid
Cell Wall
Sodium
Peptide Hydrolases

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Chromosomal DNA preparation from yeasts of biotechnological importance. / Palágyi, Zs; Nagy, Á; Papp, T.; Ferenczy, L.; Vágvölgyi, C.

In: Biotechnology Techniques, Vol. 10, No. 8, 1996, p. 565-568.

Research output: Contribution to journalArticle

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