Chromatographic determination of nanogram levels of 2-(methylpropargylamino)-1-phenylpropane (jumex,deprenyl) in plasma

Z. Juvancz, I. Ràtonyi, A. Tòth, M. Vajda

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Abstract

A quantitative capillary gas chromatographic method for the determination of 2-(methylpropargylamino)-1-phenylpropane (Jumex, Deprenyl) in plasma has been developed. The main problems in the development of the method were (1) very low therapeutic doses and consequently the necessity of very high sensitivity, and (2) the volatility of the free base form of Deprenyl. Various methods for the extraction of the compound and its pre-purification were tried, such as direct extraction from alkaline solutions, ion-pair extraction from acid solutions, extraction using Extrelut and Celite columns, and preconcentration and extraction on reversed-phase columns. As these methods turned out to be unsatisfactory, steam distillation of the free base was used for preseparation, and direct extraction of the distillate using a small amount of chloroform was used as a concentration step. The apparatus of Schulek, Constructed originally for the determination of CO2, was used for the steam distillation step. The final determination was carried out on a glass capillary column coated with Carbowax 20M impregnated with potassium hydroxide. A home-made all-glass direct injector and a Perkin-Elmer nitrogen-phosphorus detector were used. The quantitation limit of the method is 3 ng peprenyl per ml plasma.

Original languageEnglish
Pages (from-to)363-369
Number of pages7
JournalJournal of Chromatography A
Volume286
Issue numberC
DOIs
Publication statusPublished - Mar 23 1984

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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